Objectif "Electrical phenomena in plants were first documented in the 1800s, yet progress in this area has been slow. There is evidence that most plants produce electrical signals that travel long distances within the plant. This electrical activity is triggered by biotic and abiotic stimuli, supporting the notion that excitability is a robust biological trait in plants. Major objectives of this project are to characterize waveform parameters (e.g. duration, amplitude) as well as dynamic parameters (e.g. velocity) of electrical signals induced by wounding in Arabidopsis thaliana. To this end, we will develop a technique that uses aphid stylets severed from feeding aphids with a highly focused radio-frequency pulse. With this approach we will register single electrical signals that travel along the phloem, a major site of electrical activity. These intracellular recordings will be paired with surface recordings obtained simultaneously for the same signals. Wounds will be inflicted with a collimated laser beam, an approach that does not mechanically disturb the plant and is therefore suitable for electrophysiology, while allowing controlling location and size of the wound. Laser wounding induces an electrical signal similar in waveform and duration to that induced by mechanical wounding (crushing). Both wounding methods dramatically increase expression of JAZ10, a jasmonate response gene that is commonly used as a marker of the wound response. The wound-induced electrical signal will be also investigated in loss-of-function mutant plants that either do not produce the jasmonate response (aos mutant) or that do not respond to jasmonate (coi1-1 mutant). Whether aphid infestation remodels the wound-induced electrical signal will also be tested. This is an interdisciplinary project that brings together an expert in the plant's wound response and an expert in animal electrophysiology, to create new knowledge in a neglected area of Biology." Champ scientifique engineering and technologyelectrical engineering, electronic engineering, information engineeringinformation engineeringtelecommunicationsradio technologyradio frequencynatural sciencesphysical sciencesopticslaser physics Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2011-IIF - Marie Curie Action: "International Incoming Fellowships" Appel à propositions FP7-PEOPLE-2011-IIF Voir d’autres projets de cet appel Régime de financement MC-IIF - International Incoming Fellowships (IIF) Coordinateur JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG Contribution de l’UE € 74 820,80 Adresse SANDERRING 2 97070 Wuerzburg Allemagne Voir sur la carte Région Bayern Unterfranken Würzburg, Kreisfreie Stadt Type d’activité Higher or Secondary Education Establishments Contact administratif Christian Gloggengiesser (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire UNIVERSITE DE LAUSANNE Participation terminée Suisse Contribution de l’UE € 165 634,27 Adresse QUARTIER UNIL CENTRE - BATIMENT UNICENTRE 1015 LAUSANNE Voir sur la carte Région Schweiz/Suisse/Svizzera Région lémanique Vaud Type d’activité Higher or Secondary Education Establishments Contact administratif Edward Farmer (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée