Cel Recent studies have shown dysregulation of microRNAs (miRNAs) in the mucosa of Crohn’s disease (CD) patients, suggesting a role for miRNAs as contributors to inflammatory bowel disease (IBD) susceptibility. The scientists from the host institution previously showed that ileal lesions in CD patients are colonized by pathogenic adherent-invasive Escherichia coli (AIEC), which are able to invade and to replicate within intestinal epithelial cells, and that functional autophagy is required to restrain AIEC replication. The overall hypothesis of this proposal is that miRNA pathway contributes to innate immune responses to AIEC colonization and/or inflammatory activities. The goal of our study is to determine the microRNAs dysregulated in host cells upon infection with AIEC, and the mechanism through which they contribute to responses of the host. In particular, three objectives will be addressed in this project: 1) To determine the potential role and mechanism by which miRNAs modulate the host responses of intestinal epithelial cells (IECs) to AIEC; 2) To investigate the regulation of CEACAM6, a host receptor for AIEC, by miRNAs in IECs during pathophysiological state, and in host responses to AIEC infection; and 3) To test the hypothesis that AIEC impair autophagy to replicate in host cells by modulating expression of host miRNAs. I propose to use IEC culture models of AIEC infection accompanied with bioinformatics tools to identify the miRNAs which are regulators of the key signaling pathways that modulate cross-talk between AIEC and host cells. The proposed studies in this project will shed light on the mechanism by which miRNAs are involved in host responses to AIEC infection, which could help to identify new biomarkers and potential therapeutic targets for IBD. Dziedzina nauki medical and health sciencesclinical medicinegastroenterologyinflammatory bowel diseasemedical and health sciencesbasic medicineimmunology Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2011-IIF - Marie Curie Action: "International Incoming Fellowships" Zaproszenie do składania wniosków FP7-PEOPLE-2011-IIF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IIF - International Incoming Fellowships (IIF) Koordynator INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Wkład UE € 193 594,80 Adres RUE DE TOLBIAC 101 75654 Paris Francja Zobacz na mapie Region Ile-de-France Ile-de-France Paris Rodzaj działalności Research Organisations Kontakt administracyjny Evelyne Bertino (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych Uczestnicy (1) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko UNIVERSITE D'AUVERGNE CLERMONT-FERRAND 1 Zakończenie uczestnictwa Francja Wkład UE Brak danych Adres BOULEVARD FRANCOIS MITTERAND 49 63001 Clermont-Ferrand Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Isabelle Rhit (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych