Final Report Summary - ROSETTE NSC SCREEN (Mechanisms of Neurogenesis from Drosophila to Human)
The goals of this project were to establish an in vitro method to examine mechanisms of human brain development in vivo. This project has been extremely successful in that it has lead to the establishment of an entirely novel 3D method for generation of human brain tissue. Madeline has successfully established human stem cell work in the lab, and has used pluripotent stem cells to differentiate so-called cerebral organoids. The organoids have been successfully used to examine mechanisms of human neural stem cell division and differentiation as set out in the initial aims of the project. The project did not lead to a screen for novel candidate genes, as initially planned, since the new 3D method does not lend itself to high throughput screening. However, it is instead highly useful for examining complex mechanisms of brain development in a hypothesis driven manner.
These organoids will continue to be used in the lab and the method is currently being established and successfully implemented in many external laboratories through collaborations and independent research projects.
These organoids will continue to be used in the lab and the method is currently being established and successfully implemented in many external laboratories through collaborations and independent research projects.