Objectif Grapevine is one of the most important crops in Europe for production and cultivated area. Ripening is a process that increases flavor and aroma due to the accumulation of anthocyanin and aromatic compounds in berries but it limits transport, store and manipulation. Grape ripening follows a double sigmoid curve characterized for a first volume increase of the berry and a second phase, also called véraison, of accumulation in sugars, softening, coloring and a second increase in size. Grapevine is traditionally classified as a no climacteric fruit supposing an ethylene independent pathway while new recently insights showed a common genetic mechanism between climacteric and no-climacteric fruits where ethylene plays a fundamental role (Lin et al., 2009).The final step in ethylene signaling is regulated by transcription factors called Ethylene Responsive Factors (ERFs) that are involved in fruit ripening and stress processes. It was demonstrated that they bind to a specific GCC-box and modulate genes expression (Fujimoto et al., 2000) and they have been described in tomato, Arabidopsis and other fruits. At FEM, the two interesting ERFs, VvERF045 and VvERF063, have been identified showing the most interesting expression profile correlated to véraison in grape. The first accumulated its transcripts after veráison while the second one showed a clear decrease in expression. Also transgenic plants overexpressing VvERF045 were obtained.The main purpose of the project is a first characterization from a molecular, morphological and biochemical point of view of these transgenic plants to observe the main phenotypical alterations. The research is further focused on the identification of the targets of VvERF045 to better know. We will focus on the most interesting genes resulting for a further association analysis study on a germplasm bank that is present in the research center. This results should help for genetic studies on ripening and breeding purposes. Champ scientifique agricultural sciencesagriculture, forestry, and fisheriesagriculturehorticulturefruit growingnatural scienceschemical sciencesorganic chemistryaromatic compoundsnatural scienceschemical sciencesorganic chemistryaliphatic compounds Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants" Appel à propositions FP7-PEOPLE-2011-CIG Voir d’autres projets de cet appel Régime de financement MC-CIG - Support for training and career development of researcher (CIG) Coordinateur FONDAZIONE EDMUND MACH Contribution de l’UE € 100 000,00 Adresse VIA EDMONDO MACH 1 38098 San Michele All'Adige Italie Voir sur la carte Région Nord-Est Provincia Autonoma di Trento Trento Type d’activité Higher or Secondary Education Establishments Contact administratif Claudio Moser (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
