Objectif During meiosis, homologous recombination plays a mechanical role by connecting homologous chromosomes, thus allowing proper chromosome segregation during the first meiotic division. In most species, the absence of meiotic recombination leads to sterility. In addition, recombination generates new combinations of alleles, increases genome diversity and plays a major role in genome evolution.Meiotic recombination is initiated by the programmed induction of DNA double-strand breaks (DSBs), but how these events are controlled at the molecular level and how they are constrained by selective pressures during evolution is not understood.Our recent historical discovery that the Prdm9 gene controls the localization of recombination in the mouse and human genomes revolutionizes our view on this process, with one main unanticipated finding: the highly dynamic and fast evolution of Prdm9 and of meiotic DSB sites in the genome. Understanding meiotic recombination clearly requires the development of novel approaches to analyze this process from both molecular and evolutionary perspectives.To this aim, we will develop an extensive analysis of Prdm9 as its activity, role, regulators and sites of action in the genome need to be identified and understood in order to gain insight into its dynamics and evolution.We will develop a unique and challenging strategy to overcome the limitation of laboratory mice and pioneer the analysis of Prdm9 and recombination activity in wild mice.We thus aim at making a breakthrough in the field by bringing molecular genetics and evolutionary biology together, to grasp the significance of meiotic recombination for genome evolution and sexual reproduction in eukaryotes. Champ scientifique natural sciencesbiological sciencesmolecular biologymolecular geneticsnatural sciencesbiological sciencesevolutionary biologynatural sciencesbiological sciencesgeneticsheredity Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-AG-LS1 - ERC Advanced Grant - Molecular and Structural Biology and Biochemistry Appel à propositions ERC-2012-ADG_20120314 Voir d’autres projets de cet appel Régime de financement ERC-AG - ERC Advanced Grant Institution d’accueil CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Contribution de l’UE € 2 491 899,00 Adresse RUE MICHEL ANGE 3 75794 Paris France Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Chercheur principal Bernard Robert De Massy (Dr.) Contact administratif Jocelyn Mere (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS France Contribution de l’UE € 2 491 899,00 Adresse RUE MICHEL ANGE 3 75794 Paris Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Chercheur principal Bernard Robert De Massy (Dr.) Contact administratif Jocelyn Mere (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée