Objective Transcriptional regulation of protein coding genes in eukaryotic cells requires a complex interplay of sequence-specific DNA-binding factors, co-activators, general transcription factors (GTFs), RNA polymerase II and the epigenetic status of target sequences. Nuclear transcription complexes function as large multiprotein assemblies and are often composed of functional modules. The regulated decision-making that exists in cells governing the assembly and the allocation of factors to different transcription complexes to regulate distinct gene expression pathways is not yet understood. To tackle this fundamental question, we will systematically analyse the regulated biogenesis of transcription complexes from their sites of translation in the cytoplasm, through their assembly intermediates and nuclear import, to their site of action in the nucleus. The project will have four main Aims to decipher the biogenesis of transcription complexes:I) Investigate their co-translation-driven assemblyII) Determine their cytoplasmic intermediates and factors required for their assembly pathwaysIII) Uncover their nuclear importIV) Understand at the single molecule level their nuclear assembly, dynamics and action at target genesTo carry out these aims we propose a combination of multidisciplinary and cutting edge approaches, out of which some of them will be high-risk taking, while others will utilize methods routinely run by the group. The project builds on several complementary expertise and knowledge either already existing in the group or that will be implemented during the project. At the end of the proposed project we will obtain novel results extensively describing the different steps of the regulatory mechanisms that control the assembly and the consequent gene regulatory function of transcription complexes. Thus, we anticipate that the results of our research will have a major impact on the field and will lead to a new paradigm for contemporary metazoan transcription. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNA Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-AG-LS1 - ERC Advanced Grant - Molecular and Structural Biology and Biochemistry Call for proposal ERC-2013-ADG See other projects for this call Funding Scheme ERC-AG - ERC Advanced Grant Host institution CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE EU contribution € 2 500 000,00 Address Rue Laurent Fries 1 67404 Illkirch Graffenstaden France See on map Region Grand Est Alsace Bas-Rhin Activity type Research Organisations Principal investigator Laszlo Tora (Dr.) Administrative Contact Steve Brooks (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE France EU contribution € 2 500 000,00 Address Rue Laurent Fries 1 67404 Illkirch Graffenstaden See on map Region Grand Est Alsace Bas-Rhin Activity type Research Organisations Principal investigator Laszlo Tora (Dr.) Administrative Contact Steve Brooks (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data