Obiettivo Three-dimensional (3D) fluorescence imaging microscopy of individual living cells is an essential tool for cellular biology, pathology and the study of infection and virulence therein. However, today, one critical constraint of established techniques is that samples must be stabilised by attachment to an optically transparent surface, thereby completely precluding their use for non-adherent cell types. The severity of this limitation becomes clear when one considers that for basic- and biomedical-research, cell-based assay and cell-diagnostic applications some of the most important targets are non-adherent cells, for example, stem cells, systemic cancer cells and lymphocytes. We therefore propose a completely new 3D imaging strategy targeted specifically at live, non-adherent cells. The core technology combines proprietary state of the art hardware for suspended cell manipulation with super high-speed dynamic imaging methods. Around this, our consortium draws together unique expertise from four SME and three academic teams providing the necessary critical-mass to industrialize this methodology as 1) a routine research-bench tool, and 2) a high-throughput, high-content imaging device AUTOMATION (automated tomographic analysis station).Towards these goals completed pilot studies already demonstrate the feasibility for hardware and mathematical development, which will pave the way towards prototype design, and construction. As such, AUTOMATION envisages an altogether new micro-imaging technology; enabling hands-off, rapid, quantitative 3D reconstruction, as never before realised. While offering the promise of benefits across multiple areas of FP6 biomedical research, the primarily technological aims of AUTOMATION place it outside the FP6 thematic priority. However, combining ambitious goals with new and exclusive technologies the NEST-Adventure programme wholly embraces the essence of our initiative. Campo scientifico social sciencessociologyindustrial relationsautomationnatural sciencesphysical sciencesopticsmicroscopymedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesclinical medicineoncologymedical and health sciencesbasic medicinepathology Parole chiave fluorescence microscopy tomography Programma(i) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) NEST-2003-1 - Adventure activities Invito a presentare proposte FP6-2003-NEST-A Vedi altri progetti per questo bando Meccanismo di finanziamento STREP - Specific Targeted Research Project Coordinatore INSTITUT PASTEUR Contributo UE Nessun dato Indirizzo 28-25, Rue de Docteur Roux 2 PARIS Francia Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (5) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto ANDOR TECHNOLOGY LTD Regno Unito Contributo UE Nessun dato Indirizzo Millennium Way 9 Springvale Business Park BELFAST Mostra sulla mappa Costo totale Nessun dato ECOLE NORMALE SUPÉRIEURE DE CACHAN Francia Contributo UE Nessun dato Indirizzo Avenue du President Wilson 61 CACHAN Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato EVOTEC TECHNOLOGIES GMBH Germania Contributo UE Nessun dato Indirizzo Schnackenburgallee 114 HAMBURG Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato SCIENTIFIC VOLUME IMAGING BV Paesi Bassi Contributo UE Nessun dato Indirizzo Alexanderlaan 14 HILVERSUM Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato TEKNILLINEN KORKEAKOULU Finlandia Contributo UE Nessun dato Indirizzo Otakaari 1 1000 ESPOO Mostra sulla mappa Costo totale Nessun dato
