Objectif The project aims to investigate the relationships between the structure of periodic oligosaccharides and their selective fermentation in the human gut. Specifically, the influence of molecular weight, monosaccharidecomposition and linkage type will be evaluated. Fermentation selectivity will be evaluated in faecal batch cultures using fluorescent in site hybridisation to monitor changes in bacterial populations. Data from such studies will bemuse to calculate a periodic index to allow quantitative comparisons of differing carbohydrate structures. Bacterial community profiles will be characterised by denaturing gradient gel electrophoresis. Bacterial metabolic end products such as short chain fatty acids and gas will be measured. To rationalise the metabolism of specificoligosaccharide structures, selected glycosidase enzymes will be isolated from gut bacteria and characterised. In addition, the presence of oligosaccharide transport systems will investigate. Information from the project will underpin functional food development activities around Europe and in several funded projects. Champ scientifique natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural scienceschemical scienceselectrochemistryelectrophoresisnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesengineering and technologyindustrial biotechnologybioprocessing technologiesfermentation Mots‑clés Prebiotics functional food ingredients Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Appel à propositions FP6-2002-MOBILITY-5 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur UNIVERSITY OF READING Contribution de l’UE Aucune donnée Adresse Whiteknights House, Whiteknights READING Royaume-Uni Voir sur la carte Coût total Aucune donnée
