Obiettivo Chirality is a key factor in the efficacy of many drugs and the production of single enantiomers of chiral intermediates has therefore become increasingly important. Biocatalysis offers high enantioselectivity and regioselectivity in chiral synthesis through enzyme-catalyzed reactions and thus has an important advantage over chemical synthesis. Molecular genomic data is an unprecedented resource of enzymes for biocatalysis, but rational and effective methodologies must be established to realize the full potential of these resources. This project will focus on the discovery of novel enzymes, from both public and proprietary eubacterial genomes, in particular novel alcohol dehydrogenases, cytochrome P450 monooxygenases and amino acid modifying enzymes for use in established and innovative processes for chiral synthesis.The DATAGENOM project extends from genome analysis, through cloning, expression, enzyme production, screening and protein engineering, to the enzymatic production of chiral biomolecules. The design of the project takes advantage of broad funnel-approach starting with innovative data-mining and processing of a large number of genes to ensure high flow- through in the process and rational selection of best enzyme candidates. The specific combination of expertise and design of the research project is aimed at high success-rate for the development of successful biocatalysts. Emphasis will be put on effective bioinformatics analysis to minimize the requirement for the more laborious "wet chemistry" analysis as well as development of optimised vector-host systems for efficient gene expression and enzyme production. Rational protein engineering or directed molecular evolution will be employed in order to obtain more robust variants, new substrate preferences or enhanced enantiomeric selectivity. Campo scientifico natural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencescatalysisbiocatalysisnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Parole chiave Genomes chiral biomolecules enzymes metagenome Programma(i) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) LSH-2002-1.2.5-4 - Exploiting post genomics to produce optically active therapeutic biomolecules by biocatalysis Invito a presentare proposte FP6-2002-LIFESCIHEALTH Vedi altri progetti per questo bando Meccanismo di finanziamento STREP - Specific Targeted Research Project Coordinatore PROKARIA EHF Contributo UE Nessun dato Indirizzo Gylfaflot 5 REYKJAVIK Islanda Mostra sulla mappa Costo totale Nessun dato Partecipanti (8) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto INGENZA LTD. Regno Unito Contributo UE Nessun dato Indirizzo Caslte street, 39 EDINBURGH Mostra sulla mappa Costo totale Nessun dato LUNDS UNIVERSITET Svezia Contributo UE Nessun dato Indirizzo Paradisgatan 5c LUND Mostra sulla mappa Costo totale Nessun dato UNIVERSITAET STUTTGART Germania Contributo UE Nessun dato Indirizzo Keplerstrasse 7 STUTTGART Mostra sulla mappa Costo totale Nessun dato UNIVERSIDAD DE OVIEDO Spagna Contributo UE Nessun dato Indirizzo Plaza de Riego no 4 OVIEDO Mostra sulla mappa Costo totale Nessun dato DEGUSSA AG Germania Contributo UE Nessun dato Indirizzo Benningsenplatz 1 HANAU-WOLFGANG Mostra sulla mappa Costo totale Nessun dato BIOINFOBANK INSTITUTE Polonia Contributo UE Nessun dato Indirizzo Limanowskiego 24/a POZNAN Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato JFC - JUELICH FINE CHEMICALS GMBH Germania Contributo UE Nessun dato Indirizzo Rudolf-Schulten-Strasse 5 JUELICH Mostra sulla mappa Costo totale Nessun dato CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL Germania Contributo UE Nessun dato Indirizzo OLSHAUSENSTRASSE 40 KIEL Mostra sulla mappa Costo totale Nessun dato