P4SB – From Plastic waste to Plastic value using Pseudomonas putida Synthetic Biology

Project Information

P4SB

Grant agreement ID: 633962

DOI 10.3030/633962

Project closed

EC signature date 11 February 2015

Start date 1 April 2015

End date 31 March 2019

Funded under

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies – Biotechnology

Total cost € 7 056 968,75

EU contribution € 7 056 968,50

Coordinated by

RHEINISCH-WESTFAELISCHE TECHNISCHE HOCHSCHULE AACHEN
Germany

Deliverables

Websites, patent fillings, videos etc. (3)

Interim report on scientific dissemination and exploitation of results incl. risk assessment, ethical, societal, and intellectual property aspects

Related to task 83 First report on scientific dissemination and exploitation of project results publications of original papers in peer reviewed scientific journals presentations on workshops and seminars Risk assessment ethical societal and intellectual property aspects are an integral part of the project and therefore dedicated tasks to assess safety ethical and IP issues and to engage with public and journalists have been foreseen

Final report on scientific dissemination and exploitation of results incl. risk assessment, ethical, societal and intellectual property aspects

Related to task 8.3 - Updated report on scientific dissemination and exploitation of project results (publications of original papers in peer reviewed scientific journals, presentations on workshops, and seminars). Conclusion of the risk assessment, ethical, societal and intellectual property aspects, which are an integral part of the project.

P4SB-project website with corporate identity

Related to task 81 A website with open access for networks relevant platforms and stakeholders It will also show the project corporate identity

Open Research Data Pilot (2)

Initial Data Management Plan

Related to task 82 In the initial DMP the consortium will determine and explain which of the research data generated during the project phase will be made open The DMP will be written describing naming conventions central storage and publically used naming conventions such as SBOL MIAME and SBML

Interim Data Management Plan

Related to task 82 After the first half of the project lifetime the initial DMP will be revised and adapted to new insights

Publications

Peer reviewed articles (41)

Unraveling 1,4-Butanediol Metabolism in Pseudomonas putida KT2440

Author(s): Wing-Jin Li, Tanja Narancic, Shane T. Kenny, Paul-Joachim Niehoff, Kevin O’Connor, Lars M. Blank, Nick Wierckx
Published in: Frontiers in Microbiology, Issue 11, 2020, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2020.00382

Phasin interactome reveals the interplay of PhaF with the polyhydroxyalkanoate transcriptional regulatory protein PhaD in Pseudomonas putida

Author(s): Natalia A. Tarazona, Ana M. Hernández‐Arriaga, Ryan Kniewel, M. Auxiliadora Prieto
Published in: Environmental Microbiology, Issue 22/9, 2020, Page(s) 3922-3936, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15175

Exploiting the Natural Diversity of RhlA Acyltransferases for the Synthesis of the Rhamnolipid Precursor 3-(3-Hydroxyalkanoyloxy)Alkanoic Acid.

Author(s): Andrea Germer; Till Tiso; Conrad Müller; Beate Behrens; Christian Vosse; Karen Scholz; Matti Froning; Heiko Hayen; Lars M. Blank
Published in: Applied and Environmental Microbiology, Issue 2, 2020, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.02317-19

Engineered PQQ-Dependent Alcohol Dehydrogenase for the Oxidation of 5-(Hydroxymethyl)furoic Acid

Author(s): Matthias Wehrmann, Eslam M. Elsayed, Sebastian Köbbing, Laura Bendz, Alexander Lepak, Johannes Schwabe, Nick Wierckx, Gert Bange, Janosch Klebensberger
Published in: ACS Catalysis, Issue 10/14, 2020, Page(s) 7836-7842, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.0c01789

Recent Advances in Bioplastics: Application and Biodegradation.

Author(s): Tanja Narancic; Federico Cerrone; Niall Beagan; Kevin E. O’Connor
Published in: Polymers, Issue 12/4, 2020, Page(s) 920, ISSN 2073-4360
Publisher: MDPI
DOI: 10.3390/polym12040920

A Combined Bio-Chemical Synthesis Route for 1-Octene Sheds Light on Rhamnolipid Structure

Author(s): Till Tiso; Daniel F. Sauer; Klaus Beckerle; Christian C. Blesken; Jun Okuda; Lars M. Blank
Published in: Catalysts, Issue 10/8, 2020, Page(s) 874, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal10080874

Biocatalytic recycling of polyethylene terephthalate plastic

Author(s): Wolfgang Zimmermann
Published in: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Issue 378/2176, 2020, Page(s) 20190273, ISSN 1364-503X
Publisher: Royal Society of London
DOI: 10.1098/rsta.2019.0273

A quantitative method for proteome reallocation using minimal regulatory interventions

Author(s): Lastiri-Pancardo, Gustavo; Mercado-Hernandez, J.S; Kim, Juhyun; Jiménez, José I.; Utrilla, José
Published in: Nature Chemical Biology, Issue 5, 2020, Page(s) 1026–1033, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1101/733592

MEMOTE for standardized genome-scale metabolic model testing

Author(s): Bernhard O. Palsson; Bernhard O. Palsson; Filipe Liu; Isabel Rocha; Isabel Rocha; Andreas Dräger; José P. Faria; Nathan D. Price; Christian Lieven; Nathan E. Lewis; Nathan E. Lewis; Vassily Hatzimanikatis; Qianqian Yuan; Cheng Zhang; Siddharth Chauhan; Gregory L. Medlock; Wout van Helvoirt; Wout van Helvoirt; Beatriz García-Jiménez; Beatriz García-Jiménez; Radhakrishnan Mahadevan; Edda Klipp
Published in: Nature Biotechnology, 38(3), 272 - 276. Nature Publishing Group, Issue 5, 2020, Page(s) 272-276, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-020-0446-y

Quantification of outer membrane vesicles: a potential tool to compare response in Pseudomonas putida KT2440 to stress caused by alkanols

Author(s): Christian Eberlein, Stephan Starke, Álvaro Escobar Doncel, Francesco Scarabotti, Hermann J. Heipieper
Published in: Applied Microbiology and Biotechnology, Issue 103/10, 2019, Page(s) 4193-4201, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-019-09812-0

Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes

Author(s): Alexander P. S. Darlington, Juhyun Kim, José I. Jiménez, Declan G. Bates
Published in: Nature Communications, Issue 9/1, 2018, Page(s) 695, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-02898-6

Immediate response mechanisms of Gram-negative solvent-tolerant bacteria to cope with environmental stress: cis-trans isomerization of unsaturated fatty acids and outer membrane vesicle secretion

Author(s): Christian Eberlein, Thomas Baumgarten, Stephan Starke, Hermann J. Heipieper
Published in: Applied Microbiology and Biotechnology, Issue 102/6, 2018, Page(s) 2583-2593, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-018-8832-9

To be, or not to be biodegradable… that is the question for the bio-based plastics

Author(s): Auxiliadora Prieto
Published in: Microbial Biotechnology, Issue 9/5, 2016, Page(s) 652-657, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.12393

Prospects for harnessing biocide resistance for bioremediation and detoxification

Author(s): Siavash Atashgahi, Irene Sánchez-Andrea, Hermann J. Heipieper, Jan R. van der Meer, Alfons J. M. Stams, Hauke Smidt
Published in: Science, Issue 360/6390, 2018, Page(s) 743-746, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aar3778

About how to capture and exploit the CO 2 surplus that nature, per se, is not capable of fixing

Author(s): Manuel S. Godoy, Beatrice Mongili, Debora Fino, M. Auxiliadora Prieto
Published in: Microbial Biotechnology, Issue 10/5, 2017, Page(s) 1216-1225, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.12805

Designer rhamnolipids by reduction of congener diversity : production and characterization

Author(s): Tiso, Till; Zauter, Rabea; Tulke, Hannah; Leuchtle, Bernd; Li, Wing Jin; Behrens, Beate; Wittgens, Andreas; Rosenau, Frank; Hayen, Heiko; Blank, Lars Mathias
Published in: Microbial cell factories 16, 225 (2017). doi:10.1186/s12934-017-0838-y, Issue 9, 2017, Page(s) 14;16(1):225, ISSN 1475-2859
Publisher: BioMed Central
DOI: 10.18154/RWTH-2018-00735

Plastic waste as a novel substrate for industrial biotechnology

Author(s): Nick Wierckx, M. Auxiliadora Prieto, Pablo Pomposiello, Victor de Lorenzo, Kevin O'Connor, Lars M. Blank
Published in: Microbial Biotechnology, Issue 8/6, 2015, Page(s) 900-903, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.12312

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

Author(s): Sebastian Zobel, Ilaria Benedetti, Lara Eisenbach, Victor de Lorenzo, Nick Wierckx, Lars M. Blank
Published in: ACS Synthetic Biology, Issue 4/12, 2015, Page(s) 1341-1351, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.5b00058

Engineering Pseudomonas putida KT2440 for efficient ethylene glycol utilization

Author(s): Mary Ann Franden, Lahiru N. Jayakody, Wing-Jin Li, Neil J. Wagner, Nicholas S. Cleveland, William E. Michener, Bernhard Hauer, Lars M. Blank, Nick Wierckx, Janosch Klebensberger, Gregg T. Beckham
Published in: Metabolic Engineering, Issue 48, 2018, Page(s) 197-207, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2018.06.003

Biodegradable Plastic Blends Create New Possibilities for End-of-Life Management of Plastics but They Are Not a Panacea for Plastic Pollution

Author(s): Tanja Narancic, Steven Verstichel, Srinivasa Reddy Chaganti, Laura Morales-Gamez, Shane T. Kenny, Bruno De Wilde, Ramesh Babu Padamati, Kevin E. O’Connor
Published in: Environmental Science & Technology, 2018, ISSN 0013-936X
Publisher: American Chemical Society
DOI: 10.1021/acs.est.8b02963

Engineering Translational Resource Allocation Controllers: Mechanistic Models, Design Guidelines, and Potential Biological Implementations

Author(s): Alexander P. S. Darlington, Juhyun Kim, José I. Jiménez, Declan G. Bates
Published in: ACS Synthetic Biology, 2018, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.8b00029

Properties of alternative microbial hosts used in synthetic biology: towards the design of a modular chassis

Author(s): J. Kim, M. Salvador, E. Saunders, J. Gonzalez, C. Avignone-Rossa, J. I. Jimenez
Published in: Essays In Biochemistry, Issue 60/4, 2016, Page(s) 303-313, ISSN 0071-1365
Publisher: Portland Press, Ltd.
DOI: 10.1042/EBC20160015

Fast turbidimetric assay for analyzing the enzymatic hydrolysis of polyethylene terephthalate model substrates

Author(s): Matheus Regis Belisário-Ferrari, Ren Wei, Tobias Schneider, Annett Honak, Wolfgang Zimmermann
Published in: Biotechnology Journal, 2018, Page(s) 1800272, ISSN 1860-6768
Publisher: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/biot.201800272

Isolation and characterization of different promising fungi for biological waste management of polyurethanes

Author(s): Audrey Magnin, Lucie Hoornaert, Eric Pollet, Stéphanie Laurichesse, Vincent Phalip, Luc Avérous
Published in: Microbial Biotechnology, 2018, Page(s) 1-12, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.13346

Interfacial Activity of Phasin PhaF from Pseudomonas putida KT2440 at Hydrophobic–Hydrophilic Biointerfaces

Author(s): Aranzazu Mato, Natalia A. Tarazona, Alberto Hidalgo, Antonio Cruz, Mercedes Jiménez, Jesús Pérez-Gil, M. Auxiliadora Prieto
Published in: Langmuir, Issue 35/3, 2019, Page(s) 678-686, ISSN 0743-7463
Publisher: American Chemical Society
DOI: 10.1021/acs.langmuir.8b03036

Role of leucine zipper-like motifs in the oligomerization of Pseudomonas putida phasins

Author(s): Natalia A. Tarazona, Beatriz Maestro, Olga Revelles, Jesús M. Sanz, M. Auxiliadora Prieto
Published in: Biochimica et Biophysica Acta (BBA) - General Subjects, Issue 1863/2, 2019, Page(s) 362-370, ISSN 0304-4165
Publisher: Elsevier BV
DOI: 10.1016/j.bbagen.2018.11.002

Enzymatic recycling of thermoplastic polyurethanes: Synergistic effect of an esterase and an amidase and recovery of building blocks

Author(s): Audrey Magnin, Eric Pollet, Rémi Perrin, Christophe Ullmann, Cécile Persillon, Vincent Phalip, Luc Avérous
Published in: Waste Management, Issue 85, 2019, Page(s) 141-150, ISSN 0956-053X
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.wasman.2018.12.024

Shedding light on the black box models of the cell

Author(s): Jose Ignacio Jiménez
Published in: Microbial Biotechnology, Issue 10, 2017, Page(s) 43-45, ISSN 1751-7915
Publisher: Wiley
DOI: 10.1111/1751-7915.12489

Microbial Genes for a Circular and Sustainable Bio-PET Economy

Author(s): Salvador, Abdulmutalib, Gonzalez, Kim, Smith, Faulon, Wei, Zimmermann, Jimenez
Published in: Genes, Issue 10/5, 2019, Page(s) 373, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10050373

Molecular Insights into the Physical Adsorption of Amphiphilic Protein PhaF onto Copolyester Surfaces

Author(s): Natalia A. Tarazona, Rainhard Machatschek, Burkhard Schulz, M. Auxiliadora Prieto, Andreas Lendlein
Published in: Biomacromolecules, Issue 16 April 2019, 2019, Page(s) TBD, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.9b00069

Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

Author(s): Ren Wei, Daniel Breite, Chen Song, Daniel Gräsing, Tina Ploss, Patrick Hille, Ruth Schwerdtfeger, Jörg Matysik, Agnes Schulze, Wolfgang Zimmermann
Published in: Advanced Science, 2019, Page(s) 1900491, ISSN 2198-3844
Publisher: WILEY-VCH Verlag GmbH & Co. KGaA
DOI: 10.1002/advs.201900491

Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: how far are we?

Author(s): Ren Wei, Wolfgang Zimmermann
Published in: Microbial Biotechnology, Issue Vol. 10, 2017, 2017, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd.
DOI: 10.1111/1751-7915.12710

Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate

Author(s): Ren Wei, Wolfgang Zimmermann
Published in: Microbial Biotechnology, Issue Vol. 10, 2017, 2017, Page(s) 6, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd.
DOI: 10.1111/1751-7915.12714

Cooperation in microbial communities and their biotechnological applications

Author(s): Matteo Cavaliere, Song Feng, Orkun Soyer, Jose I. Jimenez
Published in: Environmental Microbiology, Issue 19(8), 2017, Page(s) 2949-2963, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.13767

Dissecting the Polyhydroxyalkanoate-Binding Domain of the PhaF Phasin: Rational Design of a Minimized Affinity Tag

Author(s): Aranzazu Mato, Francisco G. Blanco, Beatriz Maestro, Jesús M. Sanz, Jesús Pérez-Gil, M. Auxiliadora Prieto
Published in: Applied and Environmental Microbiology, Issue 86/12, 2020, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/AEM.00570-20

Microbial biotechnology addressing the plastic waste disaster

Author(s): Tanja Narancic, Kevin E. O'Connor
Published in: Microbial Biotechnology, 2017, Page(s) NA, ISSN 1751-7915
Publisher: John Wiley & Sons, Ltd
DOI: 10.1111/1751-7915.12775

Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements

Author(s): Rohola Hosseini, Jannis Kuepper, Sebastian Koebbing, Lars M. Blank, Nick Wierckx, Johannes H. de Winde
Published in: Microbial Biotechnology, Issue n.a., 2017, Page(s) n.a., ISSN 1751-7915
Publisher: John Wiley & Sons Ltd
DOI: 10.1111/1751-7915.12495

Let's talk about flux or the importance of (intracellular) reaction rates

Author(s): Blank, Lars Mathias
Published in: Microbial biotechnology 10(1), 28-30 (2017). doi:10.1111/1751-7915.12455, Issue 10/1, 2017, Page(s) 28-30, ISSN 1751-7915
Publisher: Wiley
DOI: 10.18154/rwth-2017-08141

Characterization of Context-Dependent Effects on Synthetic Promoters

Author(s): Sebastian Köbbing, Lars M. Blank, Nick Wierckx
Published in: Frontiers in Bioengineering and Biotechnology, Issue 8, 2020, Page(s) 1-13, ISSN 2296-4185
Publisher: Frontiers
DOI: 10.3389/fbioe.2020.00551

How to accurately assess surfactant biodegradation-impact of sorption on the validity of results

Author(s): Dorota Cierniak, Marta Woźniak-Karczewska, Anna Parus, Bogdan Wyrwas, Andreas P. Loibner, Hermann J. Heipieper, Łukasz Ławniczak, Łukasz Chrzanowski
Published in: Applied Microbiology and Biotechnology, Issue 104/1, 2020, Page(s) 1-12, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-019-10202-9

Toward Biorecycling: Isolation of a Soil Bacterium That Grows on a Polyurethane Oligomer and Monomer

Author(s): María José Cárdenas Espinosa, Andrea Colina Blanco, Tabea Schmidgall, Anna Katharina Atanasoff-Kardjalieff, Uwe Kappelmeyer, Dirk Tischler, Dietmar H. Pieper, Hermann J. Heipieper, Christian Eberlein
Published in: Frontiers in Microbiology, Issue 11, 2020, Page(s) 404, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2020.00404

Other (3)

Bio-upcycling of polyethylene terephthalate

Author(s): Till Tiso; Tanja Narancic; Ren Wei; Eric Pollet; Niall Beagan; Katja Schröder; Annett Honak; Mengying Jiang; Shane T. Kenny; Nick Wierckx; Rémi Perrin; Luc Avérous; Wolfgang Zimmermann; Kevin O’Connor; Lars M. Blank
Published in: 2020
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2020.03.16.993592

Insights into an alternative pathway for glycerol metabolism in a glycerol kinase deficient <i>Pseudomonas putida</i> KT2440

Author(s): Meg Walsh; William Casey; Shane T. Kenny; Tanja Narancic; Lars M. Blank; Nick Wierckx; Hendrik Ballerstedt; Kevin E O Connor
Published in: bioRxiv, Issue 1, 2019
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/567230

Memote: A community-driven effort towards a standardized genome-scale metabolic model test suite

Author(s): Lieven, Christian; Beber, Moritz E.; Olivier, Brett G.; Bergmann, Frank T.; Ataman, Meric; Babaei, Parizad; Bartell, Jennifer A.; Blank, Lars M.; Chauhan, Siddharth; Correia, Kevin; Diener, Christian; Dräger, Andreas; Ebert, Birgitta E.; Edirisinghe, Janaka N.; Faria, Jose P.; Feist, Adam; Fengos, Georgios; Fleming, Ronan M. T.; García-Jiménez, Beatriz; Hatzimanikatis, Vassily; van Helvoirt, Wo
Published in: Issue 5, 2018
Publisher: bioRxiv
DOI: 10.1101/350991

Thesis and dissertations (1)

Development of synthetic biology tools for Pseudomonas putida

Author(s): Köbbing, Sebastian
Published in: Aachen : Apprimus Verlag, Applied microbiology 23, 1 Online-Ressource (XVI, 162 Seiten) : Illustrationen, Diagramme (2020). doi:10.18154/RWTH-2020-09053 = Dissertation, RWTH Aachen University, 2020, Issue 23, 2020, Page(s) 1-189, ISBN 978-3-86359-899-0
Publisher: Apprimus Verlag
DOI: 10.18154/RWTH-2020-09053

Book chapters (3)

Plastic Biodegradation: Challenges and Opportunities

Author(s): Nick Wierckx, Tanja Narancic, Christian Eberlein, Ren Wei, Oliver Drzyzga, Audrey Magnin, Hendrik Ballerstedt, Shane T. Kenny, Eric Pollet, Luc Avérous, Kevin E. O’Connor, Wolfgang Zimmermann, Hermann J. Heipieper, Auxiliadora Prieto, José Jiménez, Lars M. Blank
Published in: Handbook of Hydrocarbon and Lipid Microbiology, 2018, Page(s) 1-29, ISBN 978-3-319-44535-9
Publisher: Springer International Publishing
DOI: 10.1007/978-3-319-44535-9_23-1

Biogenesis of Medium-Chain-Length Polyhydroxyalkanoates

Author(s): Ryan Kniewel, Olga Revelles Lopez, M. Auxiliadora Prieto
Published in: Biogenesis of Fatty Acids, Lipids and Membranes, Issue 02 February 2017, 2017, Page(s) 1-25, ISBN 978-3-319-43676-0
Publisher: Springer International Publishing
DOI: 10.1007/978-3-319-43676-0_29-1

Rhamnolipids: Production, Performance, and Application

Author(s): Till Tiso, Stephan Thies, Michaela Müller, Lora Tsvetanova, Laura Carraresi, Stefanie Bröring, Karl-Erich Jaeger, Lars Mathias Blank
Published in: Handbook of Hydrocarbon and Lipid Microbiology, 2017, Page(s) 1-37, ISBN 978-3-319-31421-1
Publisher: Springer International Publishing
DOI: 10.1007/978-3-319-31421-1_388-1

Conference proceedings (4)

Synthetic promoter stacking: When two plus two equals three

Author(s): Köbbing, Sebastian; Wierckx, Nick; Blank, Lars Mathias
Published in: 4th Meeting in Applied Synthetic Biology in Europe, Toulouse, France, 2018-10-24 - 2018-10-26, Issue 1, 2018
Publisher: European Federation of Biotechnology

Synthetic promoter libraries, a powerful tool for tuning gene expression

Author(s): Köbbing, Sebastian; Wynands, Benedikt Niklas Daniel; Blank, Lars Mathias; Wierckx, Nick
Published in: 3rd Congress on Applied Synthetic Biology in Europe, Lisbon, Portugal, 2016-02-22 - 2016-02-24, Issue 1, 2016
Publisher: European Federation of Biotechnology

Identification of reliable genomic landing sites for heterologous constructs inPseudomonas putida KT2440

Author(s): Köbbing, Sebastian; Blank, Lars M.; Wierckx, Nick
Published in: 2nd Pseudomonas Grassroots Meeting, PGM-2, Leiden, Netherlands, 2019-11-07 - 2019-11-08, Issue 1, 2019
Publisher: University of Leiden

Development and application of molecular tools for Pseudomonas

Author(s): Köbbing, Sebastian; Wierckx, Nick; Blank, Lars M.
Published in: 1st Pseudomonas Grassroots Meeting, Frankfurt am Main, Germany, 2018-11-08 - 2018-11-09, Issue 1, 2018
Publisher: DECHEMA