Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols (ROBOX)

Livrables

Completion of benchmarking protocol and flowsheets established for the case studies
Stakeholder analysis
Identification of genes predicted to encode relevant robust BVMOs and oxidases
Public website and newsletter, first press releases on the project scopes
Overall analysis of environmental benefits and techno-economics of the processes and platform
Structural elucidation of relevant of BVMOs e.g. at least 3 new enzyme structures

Structural elucidation of relevant of BVMOs eg at least 3 new enzyme structures

Project leaflet in several languages
Communication and dissemination plan

Quorum sensing technology and micro fluidics platform available
Optimized enzyme production processes for ROBOX enzymes in bioreactor including DSP
Set of P450 enzymes with improved performance for hydroxylation of aromatics, alkenes and APIs and a set of API-sensors to enable P450 improvement
First generation production strains for robust BVMOs
First generation production strains for microbial and human P450s
96-well capillary electrophoreses screening system and first flow cytometer based screening system available
Generation of a first flow cytometer based screening system for quantification of one product in the BM3 demonstration process through fluorogenic sensors
New vector and host systems for enzyme coexpression
Biocatalytic and structural exploration of P450-AX as well as generation of improved variants

Installation of advisory boards

Publications

Exploring the Substrate Scope of Baeyer-Villiger Monooxygenases with Branched Lactones as Entry towards Polyesters

Auteurs: Marie A. F. Delgove, Maximilian J. L. J. Fürst, Marco W. Fraaije, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Publié dans: ChemBioChem, 2017, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700427

Production of diclofenac metabolites by applying cytochrome P450 technology

Auteurs: Iwona Kaluzna, Jan Brummund, and Martin Schuermann
Publié dans: Chimica Oggi - Chemistry Today, Numéro vol. 35(6) November/December 2017, 2017, Page(s) 55-58, ISSN 0392-839X
Éditeur: Teknoscienze s.r.l.

Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution

Auteurs: Guangyue Li, Maximilian J. L. J. Fürst, Hamid Reza Mansouri, Anna K. Ressmann, Adriana Ilie, Florian Rudroff, Marko D. Mihovilovic, Marco W. Fraaije, Manfred T. Reetz
Publié dans: Organic & Biomolecular Chemistry, Numéro 15/46, 2017, Page(s) 9824-9829, ISSN 1477-0520
Éditeur: Royal Society of Chemistry
DOI: 10.1039/C7OB02692G

Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes

Auteurs: Elvira Romero, J. Rubén Gómez Castellanos, Giovanni Gadda, Marco W. Fraaije, Andrea Mattevi
Publié dans: Chemical Reviews, Numéro 118/4, 2018, Page(s) 1742-1769, ISSN 0009-2665
Éditeur: American Chemical Society
DOI: 10.1021/acs.chemrev.7b00650

Polycyclic Ketone Monooxygenase (PockeMO): A Robust Biocatalyst for the Synthesis of Optically Active Sulfoxides

Auteurs: Gonzalo de Gonzalo, Maximilian Fürst, Marco Fraaije
Publié dans: Catalysts, Numéro 7/12, 2017, Page(s) 288, ISSN 2073-4344
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal7100288

Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase–cyclohexanone monooxygenase fusions

Auteurs: Friso S. Aalbers, Marco W. Fraaije
Publié dans: Applied Microbiology and Biotechnology, Numéro 101/20, 2017, Page(s) 7557-7565, ISSN 0175-7598
Éditeur: Springer Verlag
DOI: 10.1007/s00253-017-8501-4

Application of a thermostable Baeyer-Villiger monooxygenase for the synthesis of branched polyester precursors

Auteurs: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M.A. De Wildeman
Publié dans: Journal of Chemical Technology & Biotechnology, 2018, ISSN 0268-2575
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5623

Biocatalytic Properties and Structural Analysis of Eugenol Oxidase from Rhodococcus jostii RHA1: A Versatile Oxidative Biocatalyst

Auteurs: Quoc-Thai Nguyen, Gonzalo de Gonzalo, Claudia Binda, Ana Rioz-Martínez, Andrea Mattevi, Marco W. Fraaije
Publié dans: ChemBioChem, Numéro 17/14, 2016, Page(s) 1359-1366, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600148

Overriding Traditional Electronic Effects in Biocatalytic Baeyer–Villiger Reactions by Directed Evolution

Auteurs: Guangyue Li, Marc Garcia-Borràs, Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk, Manfred T. Reetz
Publié dans: Journal of the American Chemical Society, Numéro 140/33, 2018, Page(s) 10464-10472, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.8b04742

Toward Upscaled Biocatalytic Preparation of Lactone Building Blocks for Polymer Applications

Auteurs: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Publié dans: Organic Process Research & Development, Numéro 22/7, 2018, Page(s) 803-812, ISSN 1083-6160
Éditeur: American Chemical Society
DOI: 10.1021/acs.oprd.8b00079

High performing immobilized Baeyer-Villiger monooxygenase and glucose dehydrogenase for the synthesis of Ɛ-caprolactone derivative

Auteurs: Marie A.F. Delgove, Daniela Valencia, Jordi Solé, Katrien V. Bernaerts, Stefaan M.A. De Wildeman, Marina Guillén, Gregorio Álvaro
Publié dans: Applied Catalysis A: General, Numéro 572, 2018, Page(s) 134-141, ISSN 0926-860X
Éditeur: Elsevier BV
DOI: 10.1016/j.apcata.2018.12.036

A prospective life-cycle assessment (LCA) of monomer synthesis: Comparison of biocatalytic and oxidative chemistry

Auteurs: Marie A. F. Delgove, Achille-B. Laurent, John M. Woodley, Stefaan M. A. De Wildeman, Katrien V. Bernaerts, Yvonne van der Meer
Publié dans: ChemSusChem, 2019, ISSN 1864-5631
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.201900007

A hydroquinone-specific screening system for directed P450 evolution

Auteurs: Alexandra M. Weingartner, Daniel F. Sauer, Gaurao V. Dhoke, Mehdi D. Davari, Anna Joëlle Ruff, Ulrich Schwaneberg
Publié dans: Applied Microbiology and Biotechnology, Numéro 102/22, 2018, Page(s) 9657-9667, ISSN 0175-7598
Éditeur: Springer Verlag
DOI: 10.1007/s00253-018-9328-3

Online Measurement of Oxygen-Dependent Enzyme Reaction Kinetics

Auteurs: Murray P. Meissner, Mathias Nordblad, John M. Woodley
Publié dans: ChemBioChem, Numéro 19/1, 2018, Page(s) 106-113, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700577

Scoping Biocatalyst Performance Using Reaction Trajectory Analysis

Auteurs: Mathias Nordblad, Mafalda Dias Gomes, Murray P. Meissner, Hemalata Ramesh, John M. Woodley
Publié dans: Organic Process Research & Development, Numéro 22/9, 2018, Page(s) 1101-1114, ISSN 1083-6160
Éditeur: American Chemical Society
DOI: 10.1021/acs.oprd.8b00119

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol

Auteurs: Quoc-Thai Nguyen, Elvira Romero, Willem P. Dijkman, Suzan Pantaroto de Vasconcellos, Claudia Binda, Andrea Mattevi, Marco W. Fraaije
Publié dans: Biochemistry, Numéro 57/43, 2018, Page(s) 6209-6218, ISSN 0006-2960
Éditeur: American Chemical Society
DOI: 10.1021/acs.biochem.8b00918

Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes

Auteurs: Friso S. Aalbers, Marco W. Fraaije
Publié dans: ChemBioChem, Numéro 20/1, 2019, Page(s) 20-28, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800394

Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases

Auteurs: Maximilian JLJ Fürst, Filippo Fiorentini, Marco W Fraaije
Publié dans: Current Opinion in Structural Biology, Numéro 59, 2019, Page(s) 29-37, ISSN 0959-440X
Éditeur: Elsevier BV
DOI: 10.1016/j.sbi.2019.01.019

Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase-NADPH Oxidase Fusions for Continuous Oxidations

Auteurs: Friso S. Aalbers, Marco W. Fraaije
Publié dans: ChemBioChem, Numéro 20/1, 2019, Page(s) 51-56, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800421

What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis

Auteurs: Ángela Mourelle-Insua, Friso S. Aalbers, Iván Lavandera, Vicente Gotor-Fernández, Marco W. Fraaije
Publié dans: Tetrahedron, Numéro 75/13, 2019, Page(s) 1832-1839, ISSN 0040-4020
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.tet.2019.02.015

Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme

Auteurs: Maximilian J. L. J. Fürst, Elvira Romero, J. Rúben Gómez Castellanos, Marco W. Fraaije, Andrea Mattevi
Publié dans: ACS Catalysis, Numéro 8/12, 2018, Page(s) 11648-11656, ISSN 2155-5435
Éditeur: American Chemical Society
DOI: 10.1021/acscatal.8b03793

Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope

Auteurs: Joanne L. Porter, Selina Sabatini, Jack Manning, Michele Tavanti, James L. Galman, Nicholas J. Turner, Sabine L. Flitsch
Publié dans: Enzyme and Microbial Technology, Numéro 113, 2018, Page(s) 1-8, ISSN 0141-0229
Éditeur: Elsevier BV
DOI: 10.1016/j.enzmictec.2018.02.005

Characterisation of CYP102A25 from Bacillus marmarensis and CYP102A26 from Pontibacillus halophilus : P450 Homologues of BM3 with Preference towards Hydroxylation of Medium-Chain Fatty Acids

Auteurs: Joanne L. Porter, Jack Manning, Selina Sabatini, Michele Tavanti, Nicholas J. Turner, Sabine L. Flitsch
Publié dans: ChemBioChem, Numéro 19/5, 2018, Page(s) 513-520, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700598

A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Auteurs: Michele Tavanti, Juan Mangas-Sanchez, Sarah L. Montgomery, Matthew P. Thompson, Nicholas J. Turner
Publié dans: Organic & Biomolecular Chemistry, Numéro 15/46, 2017, Page(s) 9790-9793, ISSN 1477-0520
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c7ob02569f

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Auteurs: Astrid Weninger, Jasmin E. Fischer, Hana Raschmanová, Claudia Kniely, Thomas Vogl, Anton Glieder
Publié dans: Journal of Cellular Biochemistry, Numéro 119/4, 2018, Page(s) 3183-3198, ISSN 0730-2312
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jcb.26474

Co-immobilization of P450 BM3 and glucose dehydrogenase on different supports for application as a self-sufficient oxidative biocatalyst

Auteurs: Jordi Solé, Gloria Caminal, Martin Schürmann, Gregorio Álvaro, Marina Guillén
Publié dans: Journal of Chemical Technology & Biotechnology, Numéro 94/1, 2019, Page(s) 244-255, ISSN 0268-2575
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5770

One-pot Biocatalytic Double Oxidation of α-Isophorone for the Synthesis of Ketoisophorone

Auteurs: Michele Tavanti, Fabio Parmeggiani, Jose Rubén Gómez Castellanos, Andrea Mattevi, Nicholas John Turner
Publié dans: ChemCatChem, 2017, ISSN 1867-3880
Éditeur: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201700620

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase

Auteurs: Elvira Romero, J. Rubén Gómez Castellanos, Andrea Mattevi, Marco W. Fraaije
Publié dans: Angewandte Chemie, Numéro 128/51, 2016, Page(s) 16084-16087, ISSN 0044-8249
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201608951

Polycyclic Ketone Monooxygenase from the Thermophilic Fungus Thermothelomyces thermophila : A Structurally Distinct Biocatalyst for Bulky Substrates

Auteurs: Maximilian J. L. J. Fürst, Simone Savino, Hanna M. Dudek, J. Rúben Gómez Castellanos, Cora Gutiérrez de Souza, Stefano Rovida, Marco W. Fraaije, Andrea Mattevi
Publié dans: Journal of the American Chemical Society, Numéro 139/2, 2017, Page(s) 627-630, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.6b12246

Increasing the solubility range of polyesters by tuning their microstructure with comonomers

Auteurs: Marie A. F. Delgove, Juandré Luchies, Iris Wauters, Geert G. P. Deroover, Stefaan M. A. De Wildeman, Katrien V. Bernaerts
Publié dans: Polym. Chem., 2017, ISSN 1759-9954
Éditeur: Royal Society of Chemistry
DOI: 10.1039/C7PY00976C

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins

Auteurs: Max J.L.J. Fürst, Caterina Martin, Nikola Lončar, Marco W. Fraaije
Publié dans: Methods in Enzymology, Numéro 2018 June 9, 2018, Page(s) 151-187, ISBN 978-0-12-815148-8
Éditeur: Elsevier
DOI: 10.1016/bs.mie.2018.04.007

Flavoenzymes at the crossroads of biocatalysis and structural biology

Auteurs: Nguyen, Quoc-Thai
Publié dans: Numéro 1, 2017
Éditeur: Universityof Groningen

Droits de propriété intellectuelle

KETOREDUCTASES

Numéro de demande/publication: WO 2019012095
Date: 2017-07-14
Demandeur(s): C-LECTA GMBH

KETOREDUCTASES

Numéro de demande/publication: WO 2019012095
Date: 2017-07-14
Demandeur(s): C-LECTA GMBH

Livrables

Publications

Droits de propriété intellectuelle

Partager cette page

Télécharger