Project description DEENESFRITPL Engineering efficient enzymes for the chemical industry Chiral amines play a vital role as building blocks for pharmaceutical drugs, fine chemicals, and bioactive compounds. The use of amine dehydrogenases (AmDHs) in the synthesis of chiral amines has gained significant interest due to their potential to address the limitations of traditional chemical approaches. Funded by the European Research Council, the BIOSUSAMIN project aims to overcome limitations associated with AmDH stability, catalytic activity, and range of substrates. Researchers will use protein engineering techniques to generate new AmDHs that produce a variety of amines and derivatives with high yields and precise chemical properties. The approach aims to use renewable resources and minimise environmental impact, resulting in water as the only by-product. Show the project objective Hide the project objective Objective The objective of this project is to design and develop biocatalytic cascades, using purified enzymes in vitro, as well as biosynthetic pathways in whole cell microbial organisms. These biocatalytic cascades and biosynthetic pathways will be developed for the synthesis of chiral and achiral amines that are of particular interest for the chemical industry. The amine functionality will be introduced using amine dehydrogenases (AmDHs) as biocatalysts in the pivotal core enzymatic step. AmDHs are a new class of enzymes that have recently been obtained by protein engineering of wild-type amino acid dehydrogenases. However, only two AmDHs have been generated so far and, moreover, they show a limited substrate scope. Therefore protein engineering will be undertaken in order to expand the substrate scope of the already existing AmDHs. In addition, novel AmDHs will be generated starting from different wild-type amino acid dehydrogenases as scaffolds, whose amino acid and DNA sequences are available in databases, literature, libraries, etc. In particular, protein engineering will be focused on the specific chemical targets that are the objectives of the designed biocatalytic cascades and in addition, screening for more diverse substrates will also be carried out. Finally, the AmDHs will be used in combination with other enzymes such as alcohol dehydrogenases, oxidases, alkane monooxygenases, etc., to deliver variously functionalised amines and derivatives as final products with elevated yields, perfect chemo- regio- and stereoselectivity, enhanced atom efficiency and minimum environmental impact. Such an approach will be realised through the design of new pathways that will convert inexpensive starting materials from renewable resources, encompassing the internal recycling of redox equivalents, the use of inorganic ammonia as nitrogen source and, if necessary, only molecular oxygen as the innocuous additional oxidant. Water will be the sole by-product. Fields of science natural scienceschemical sciencesorganic chemistryalcoholsnatural scienceschemical sciencescatalysisbiocatalysisnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesearth and related environmental sciencesatmospheric sciencesclimatologyclimatic changesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-StG-2014 - ERC Starting Grant Call for proposal ERC-2014-STG See other projects for this call Funding Scheme ERC-STG - Starting Grant Host institution UNIVERSITEIT VAN AMSTERDAM Net EU contribution € 1 461 840,00 Address SPUI 21 1012WX Amsterdam Netherlands See on map Region West-Nederland Noord-Holland Groot-Amsterdam Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 461 840,00 Beneficiaries (2) Sort alphabetically Sort by Net EU contribution Expand all Collapse all UNIVERSITEIT VAN AMSTERDAM Netherlands Net EU contribution € 1 461 840,00 Address SPUI 21 1012WX Amsterdam See on map Region West-Nederland Noord-Holland Groot-Amsterdam Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 461 840,00 THE UNIVERSITY OF MANCHESTER Participation ended United Kingdom Net EU contribution € 35 430,00 Address OXFORD ROAD M13 9PL Manchester See on map Region North West (England) Greater Manchester Manchester Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 35 430,00
