How complement molecules kill bacteria

Project Information

ComBact

Grant agreement ID: 639209

DOI 10.3030/639209

Project closed

EC signature date 13 February 2015

Start date 1 March 2015

End date 29 February 2020

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 497 290,00

EU contribution € 1 497 290,00

Coordinated by

UNIVERSITAIR MEDISCH CENTRUM UTRECHT
Netherlands

Publications

Peer reviewed articles (14)

Novel Evasion Mechanisms of the Classical Complement Pathway

Author(s): Brandon L. Garcia, Seline A. Zwarthoff, Suzan H. M. Rooijakkers, Brian V. Geisbrecht
Published in: The Journal of Immunology, Issue 197/6, 2016, Page(s) 2051-2060, ISSN 0022-1767
Publisher: American Association of Immunologists
DOI: 10.4049/jimmunol.1600863

Acquisition of C1 inhibitor by Bordetella pertussis virulence associated gene 8 results in C2 and C4 consumption away from the bacterial surface

Author(s): Elise S. Hovingh, Bryan van den Broek, Betsy Kuipers, Elena Pinelli, Suzan H. M. Rooijakkers, Ilse Jongerius
Published in: PLOS Pathogens, Issue 13/7, 2017, Page(s) e1006531, ISSN 1553-7374
Publisher: plos pathogens
DOI: 10.1371/journal.ppat.1006531

Evidence for Multiple Modes of Neutrophil Serine Protease Recognition by the EAP Family of Staphylococcal Innate Immune Evasion Proteins

Author(s): Daphne A.C. Stapels, Jordan L. Woehl, Fin J. Milder, Angelino T. Tromp, Aernoud A. van Batenburg, Wilco C. de Graaf, Samuel C. Broll, Natalie M. White, Suzan H.M. Rooijakkers, Brian V. Geisbrecht
Published in: Protein Science, 2017, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3342

Molecular insights into the surface-specific arrangement of complement C5 convertase enzymes

Author(s): Evelien T. M. Berends, Ronald D. Gorham, Maartje Ruyken, Jasper A. Soppe, Hatice Orhan, Piet C. Aerts, Carla J. C. de Haas, Piet Gros, Suzan H. M. Rooijakkers
Published in: BMC Biology, Issue 13/1, 2015, ISSN 1741-7007
Publisher: BioMed Central
DOI: 10.1186/s12915-015-0203-8

The Staphylococcus aureus polysaccharide capsule and Efb-dependent fibrinogen shield act in concert to protect against phagocytosis

Author(s): Annemarie Kuipers, Daphne A. C. Stapels, Maartje Ruyken, Lleroy T. Weerwind, Suzan H. M. Rooijakkers, Jean C. Lee, Kok P. M. van Kessel, Ya-Ping Ko
Published in: Microbiology, Issue 162/7, 2016, Page(s) 1185-1194, ISSN 1350-0872
Publisher: Society for General Microbiology
DOI: 10.1099/mic.0.000293

Complement resistance mechanisms of Klebsiella pneumoniae

Author(s): Dennis J. Doorduijn, Suzan H.M. Rooijakkers, Willem van Schaik, Bart W. Bardoel
Published in: Immunobiology, Issue 221/10, 2016, Page(s) 1102-1109, ISSN 0171-2985
Publisher: Elsevier BV
DOI: 10.1016/j.imbio.2016.06.014

How the Membrane Attack Complex Damages the Bacterial Cell Envelope and Kills Gram‐Negative Bacteria

Author(s): Dennis J. Doorduijn, Suzan H. M. Rooijakkers, Dani A. C. Heesterbeek
Published in: BioEssays, Issue 41/10, 2019, Page(s) 1900074, ISSN 0265-9247
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/bies.201900074

Bacterial killing by complement requires membrane attack complex formation via surface-bound C5 convertases

Author(s): Heesterbeek, D. A.; Bardoel, B. W.; Parsons, E. S.; Bennett, I.; Ruyken, M.; Doorduijn, D. J.; Gorham, R. D.; Berends, E. T.; Pyne, A. L.; Hoogenboom, B. W.; Rooijakkers, S. H.
Published in: EMBO Journal , Article e99852. (2019) (In press)., Issue 1, 2019, ISSN 1460-2075
Publisher: EMBOpress
DOI: 10.15252/embj.201899852

Functional Characterization of Alternative and Classical Pathway C3/C5 Convertase Activity and Inhibition Using Purified Models

Author(s): Seline A. Zwarthoff, Evelien T. M. Berends, Sanne Mol, Maartje Ruyken, Piet C. Aerts, Mihály Józsi, Carla J. C. de Haas, Suzan H. M. Rooijakkers, Ronald D. Gorham
Published in: Frontiers in Immunology, Issue 9, 2018, ISSN 1664-3224
Publisher: Frontiers
DOI: 10.3389/fimmu.2018.01691

Group IIA-Secreted Phospholipase A 2 in Human Serum Kills Commensal but Not Clinical Enterococcus faecium Isolates

Author(s): Fernanda L. Paganelli, Helen L. Leavis, Samantha He, Nina M. van Sorge, Christine Payré, Gérard Lambeau, Rob J. L. Willems, Suzan H. M. Rooijakkers
Published in: Infection and Immunity, Issue 86/8, 2018, ISSN 0019-9567
Publisher: American Society for Microbiology
DOI: 10.1128/iai.00180-18

Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains

Author(s): Nienke W. M. de Jong, Manouk Vrieling, Brandon L. Garcia, Gerrit Koop, Matt Brettmann, Piet C. Aerts, Maartje Ruyken, Jos A. G. van Strijp, Mark Holmes, Ewan M. Harrison, Brian V. Geisbrecht, Suzan H. M. Rooijakkers
Published in: Journal of Biological Chemistry, Issue 293/12, 2018, Page(s) 4468-4477, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.RA117.000599

Complement and Bacterial Infections: From Molecular Mechanisms to Therapeutic Applications

Author(s): Dani A.C. Heesterbeek, Mathieu L. Angelier, Richard A. Harrison, Suzan H.M. Rooijakkers
Published in: Journal of Innate Immunity, Issue 10/5-6, 2018, Page(s) 455-464, ISSN 1662-811X
Publisher: Karger AG
DOI: 10.1159/000491439

Complement-dependent outer membrane perturbation sensitizes Gram-negative bacteria to Gram-positive specific antibiotics

Author(s): D. A. C. Heesterbeek, N. I. Martin, A. Velthuizen, M. Duijst, M. Ruyken, R. Wubbolts, S. H. M. Rooijakkers, B. W. Bardoel
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-38577-9

Let’s Tie the Knot: Marriage of Complement and Adaptive Immunity in Pathogen Evasion, for Better or Worse

Author(s): Kaila M. Bennett, Suzan H. M. Rooijakkers, Ronald D. Gorham
Published in: Frontiers in Microbiology, Issue 8, 2017, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2017.00089

Other (3)

Outer membrane permeabilization by the membrane attack complex sensitizes Gram-negative bacteria to antimicrobial proteins in serum and phagocytes

Author(s): Dani A. C. Heesterbeek, Remy M. Muts, Vincent P. van Hensbergen, Pieter de Saint Aulaire, Tom Wennekes, Bart W. Bardoel, Nina M. van Sorge, Suzan H.M. Rooijakkers
Published in: Review Commons, 2020
Publisher: Review Commons
DOI: 10.1101/2020.01.28.923243

Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7

Author(s): Dennis J Doorduijn, Bart W Bardoel, Dani AC Heesterbeek, Maartje Ruyken, Georgina Benn, Edward S Parsons, Bart Hoogenboom, Suzan HM Rooijakkers
Published in: Review Commons, 2019
Publisher: Review Commons
DOI: 10.1101/2019.12.17.877639

In vitro evolution of colistin resistance in the Klebsiella pneumoniae complex follows multiple evolutionary trajectories with variable effects on fitness and virulence characteristics

Author(s): Axel B. Janssen, Dennis J. Doorduijn, Grant Mills, Malbert R.C. Rogers, Marc J.M. Bonten, Suzan H.M. Rooijakkers, Rob J.L. Willems, Jose A. Bengoechea, Willem van Schaik
Published in: biorxiv, 2020
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2020.05.24.112334