Obiettivo The long-range goal of this project is to develop a molecular nanodevice, based on nucleic acid-protein conjugates, for the multiplexed, quantitative imaging of biomarkers in tissue samples and cultured cells.The Immuno-nanodecoder will be used for the accurate molecular characterization of skin cancer (melanoma) and glycogenosis type II cellular models and to evaluate the in vitro response to experimental therapies.The nanodevices (nanodecoders) will consist of self-assembled DNA nanostructures that can reversibly change their fluorescence signal output in response to hybridization to nucleic acid sequences, or to a specific enzymatic reaction. Each nanodevice will be coupled to a specific molecular probe, such as an antibody, peptide, or protein that uniquely recognize disease biomarkers. The coupling will allow the nanodecoder to detect biomarker presence and distribution in cells and tissues, in a layer-by-layer fashion, using optical fluorescence microscopy. The number of biomarkers that can be detected will be limited only by the capacity to design nanodecoders with differing specificities, which is essentially unlimited. The creation of nanodecoders and optimization of their function will greatly advance biomarker imaging, which currently lacks a high-throughput, convenient method for the in situ, quantitative microscopic analysis of altered tissue regions. This project will be driven by knowledge exchange and the expertise of an interdisciplinary team comprised of both early-stage and experienced university and hospital researchers.Complementary research programs, ranging from nanotechnology to molecular medicine and pathology will support each step of the developmental and applicative parts of the project towards the achievement of its objectives. Funding of this program will enable long-term, transformative collaborations that will contribute to the integration and collaboration of research groups between European Countries and key Third Countries. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsmicroscopynatural sciencesbiological sciencesgeneticsRNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programma(i) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.3. - Stimulating innovation by means of cross-fertilisation of knowledge Argomento(i) MSCA-RISE-2014 - Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) Invito a presentare proposte H2020-MSCA-RISE-2014 Vedi altri progetti per questo bando Meccanismo di finanziamento MSCA-RISE - Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) Coordinatore UNIVERSITA DEGLI STUDI DI ROMA TOR VERGATA Contribution nette de l'UE € 198 000,00 Indirizzo VIA CRACOVIA 50 00133 Roma Italia Mostra sulla mappa Regione Centro (IT) Lazio Roma Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 198 000,00 Partecipanti (2) Classifica in ordine alfabetico Classifica per Contributo netto dell'UE Espandi tutto Riduci tutto AZIENDA SANITARIA UNIVERSITARIA FRIULI CENTRALE Italia Contribution nette de l'UE € 180 000,00 Indirizzo VIA POZZUOLO 330 33100 Udine Mostra sulla mappa Regione Nord-Est Friuli-Venezia Giulia Udine Tipo di attività Research Organisations Collegamenti Contatta l’organizzazione Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 180 000,00 UNIVERSITY OF LINCOLN Regno Unito Contribution nette de l'UE € 63 000,00 Indirizzo Brayford Pool LN6 7TS Lincoln Mostra sulla mappa Regione East Midlands (England) Lincolnshire Lincolnshire Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 63 000,00 Partner (2) Classifica in ordine alfabetico Classifica per Contributo netto dell'UE Espandi tutto Riduci tutto Partner Le organizzazioni partner contribuiscono all’attuazione dell’azione, ma non sottoscrivono l’accordo di sovvenzione. TEMPLE UNIVERSITY OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION Stati Uniti Contribution nette de l'UE € 0,00 Indirizzo N BROAD STREET 1601 ROOM 406 USB 19122 PHILADELPHIA Mostra sulla mappa Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale Nessun dato Partner Le organizzazioni partner contribuiscono all’attuazione dell’azione, ma non sottoscrivono l’accordo di sovvenzione. CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET) Argentina Contribution nette de l'UE € 0,00 Indirizzo GODOY CRUZ 2290 C1425FQB Buenos Aires Mostra sulla mappa Tipo di attività Research Organisations Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 139 500,00