Periodic Reporting for period 1 - TRANSFORMER (Transformation of emerging contaminants in the aquatic environment. Fate of transformation products under multiple stress conditions)
Okres sprawozdawczy: 2015-05-11 do 2016-08-10
Task 1: Selection of target compounds
Task 2: Factorial design of the experiments at experimental stream facility
Task 3: Experiments at experiments at experimental stream facility
Task 4: Target and non-target analysis
Task 5: Analysis of the results
We learned that biofilm biodegradation ability is not affected by short term (4 days) exposure to a mixture of emerging contaminants. Multi-stress (i.e. flow intermittency and emerging contaminants exposure) conditions enhance biofilm biodegradation ability during short and medium term (13 days) exposure suggesting indirect antagonistic relationship between drought and emerging contaminants.
Exo-metabolome profiling demonstrated that different perturbations are directly reflected on biofilm released metabolites. The reported non-target profiling suggests immediate exo-metabolic response to intermittent flow conditions and emerging contaminats (4 days). 9 days after end of drought, however at constant presence of ECs, exo-metabolic profile is not any more influenced by drought perturbation and emerging contaminants are becoming major source of imbalance.
So far, the project results were disseminated via one paper:
Marko Rožman, Mira Petrović; Bquant - Novel script for batch quantification of LCMS data, MethodsX 3 2016 520-524. (DOI: 10.1016/j.mex.2016.09.001)
Two more papers on understanding interstressor interactions and biofilm system response to stress perturbations are in process of drafting and will be submitted once completed.
Furthermore, results are presented at the invited lecture: Transformation of emerging contaminants in the aquatic environment at Croatian Association of Freshwater Ecologist at the University of Zagreb.