Self-Organising Capacity of Stem Cells during Implantation and Early Post-implantation Development: Implications for Human Development

Projektinformationen

EPIROSE

ID Finanzhilfevereinbarung: 669198

DOI 10.3030/669198

Abgeschlossenes Projekt

EK-Unterschriftsdatum 21 Dezember 2015

Startdatum 1 Januar 2016

Enddatum 30 Juni 2022

Finanziert unter

• EXCELLENT SCIENCE - European Research Council (ERC)

Gesamtkosten € 2 477 951,00

EU-Beitrag € 2 477 951,00

Koordiniert durch

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
United Kingdom

Ziel

Embryonic development progresses through successive cell fate decisions and intricate three-dimensional morphogenetic transformations. Implantation is the defining event in mammalian pregnancy during which a fundamental morphogenetic transformation is initiated: the body axes are established and the embryonic germ layers created. Despite its importance, a comprehensive understanding of the molecular mechanisms, transcriptional pathways, cellular interactions, as well as the spatio-temporal development of the embryo at implantation stages is at present lacking, due to the embryo’s inaccessibility. To overcome these limitations, we generated a culture system that allows the development of mouse implanting embryos outside of the mother. This system provides the opportunity to address how architectural features and signaling events integrate to induce the emergence of the body plan. Combining this new technology with the analysis of genetically engineered mouse embryos, the aim of this research proposal is to fill the knowledge gap between pre and post-implantation development. Single cell sequencing, two-photon microscopy, high-content forward genetic screening, and modeling will be merged with a functional assessment of embryo development in vivo to reveal the determinants of implantation and early post-implantation development. This global understanding will be employed to explore the extent to which stem cells can recapitulate embryonic development, with tremendous potential for regenerative medicine. Knowledge of the cellular and molecular mechanisms that intertwine lineage specification, developmental potential, and tissue morphogenesis will offer novel insight on the pathological causes of embryo lethality and congenital disorders. The proposed studies will shed light on this crucial yet mysterious stage of development in the mouse and, by extrapolation, offer outstanding potential to advance our understanding of human development.

Wissenschaftliches Gebiet

• social sciences › media and communications › journalism
• natural sciences › biological sciences › developmental biology
• medical and health sciences › clinical medicine › obstetrics › fetal medicine
• medical and health sciences › medical biotechnology › cells technologies › stem cells
• medical and health sciences › clinical medicine › embryology

Schlüsselbegriffe

• rosette
• epiblast
• cell differentiation
• morphogenesis

Programm/Programme

• H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme

Thema/Themen

• ERC-ADG-2014 - ERC Advanced Grant

Aufforderung zur Vorschlagseinreichung

ERC-2014-ADG

Finanzierungsplan

ERC-ADG - Advanced Grant

Gastgebende Einrichtung

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

• Netto-EU-Beitrag: € 2 477 951,00
• Adresse: TRINITY LANE THE OLD SCHOOLS ; CB2 1TN Cambridge ; Vereinigtes Königreich
• Region: East of England › East Anglia › Cambridgeshire CC
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 2 477 951,00

Begünstigte (1)

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

Vereinigtes Königreich

• Netto-EU-Beitrag: € 2 477 951,00
• Adresse: TRINITY LANE THE OLD SCHOOLS ; CB2 1TN Cambridge
• Region: East of England › East Anglia › Cambridgeshire CC
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 2 477 951,00