Objectif G protein-coupled receptors (GPCRs) are the largest family of cell surface signal transducing proteins encoded by thehuman genome. They allow the cell to respond to diverse array of extracellular signals, control most (patho)physiologicalprocesses, and are currently the therapeutic target of over 30% of marketed drugs. However, GPCR drug discovery is stillcharacterised by a very high attrition rate, which reflects our inadequate understanding of the complex mechanisms ofGPCR signalling and regulation.Up until recently, understanding of GPCR function was obtained from snapshots of receptors at different points in time and amajor limitation for the study of GPCRs has been the inability to assess receptor activation and subsequent signalling eventswith high temporal (duration and frequency) or spatial (location) resolution. However, in the recent years there has been anexplosion of biophysical and imaging approaches that will allow greater temporal and spatial resolution of receptor functionthan ever before. In this project we will measure ligand binding, receptor conformational changes, G protein activation,recruitment of regulatory proteins and receptor trafficking in real time and in live cells. We will therefore obtain detailedmechanistic understanding of the dynamics of GPCR activity in health and in disease that will reveal novel interventionpoints for future, more effective receptor-based therapies.This proposal combines my expertise in the study of GPCR interacting proteins and their role in receptor signalling andtrafficking with the expertise of the Host Institution in the application of state-of-the-art imaging and biophysical approachesto study of this receptor family. As such, this project will not only broaden my research and technical skills in GPCRvisualization, but it will also result in the establishment of a unique technological platform for the study of the dynamics ofGPCR function within the Host Institution. Champ scientifique medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsgenomes Mots‑clés pharmacology membrane receptors cell biology molecular biology Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Appel à propositions H2020-MSCA-IF-2015 Voir d’autres projets de cet appel Régime de financement MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinateur THE UNIVERSITY OF NOTTINGHAM Contribution nette de l'UE € 183 454,80 Adresse University Park NG7 2RD Nottingham Royaume-Uni Voir sur la carte Région East Midlands (England) Derbyshire and Nottinghamshire Nottingham Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 183 454,80