Objective Active liquid crystals are out-of-equilibrium systems that display intriguing dynamic phenomena, arising from the interplaybetween topological defects and collective motion. Here, we propose to experimentally study for the first time (i) thedynamics of topological defects in the active colloidal nematic phase and (ii) the interplay between these topological defectsand collective dynamics, in three dimensions, at the single particle level, and with full control over the defects. To reachthese objectives, we first use a combination of advanced colloidal synthesis and surface modification techniques in order todevelop a model system of active colloidal rods of controlled size, aspect ratio and surface properties. We then confine thisnewly developed model system in microfluidic channels, where the colloidal rods form a nematic liquid crystal. Topologicaldefects will be induced in the nematic phase using specific geometric constraints, which can be varied to control thelocations and strengths of the defects, or by optical trapping techniques. Finally, we use state-of-the-art confocal microscopyand sophisticated image analysis techniques to follow, in real time and 3D real space, both the individual particle motion andthe collective dynamics in the system. Our work will thus provide experimental verification of the intriguing link betweentopological defects and collective dynamics in active liquid crystals and inspire novel theories and simulation codes capableof capturing the intrinsic complexity of coupling structure, orientation and activity. Fields of science engineering and technologymaterials engineeringliquid crystals Keywords active matter soft matter topological defects liquid crystals anisotropic colloids confocal microscopy optical trapping microfluidics Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Call for proposal H2020-MSCA-IF-2015 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Net EU contribution € 195 454,80 Address WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford United Kingdom See on map Region South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 195 454,80