Projektbeschreibung
Resistenz gegenüber onkogener Transformation
Krebszellen entstehen durch eine Kombination aus genetischen und epigenetischen Veränderungen in normalen Zellen, durch die eine Transformation begünstigt wird. Onkogene Signale lösen in manchen Zellen jedoch keine Reaktion aus, was auf die Existenz eines Mechanismus hindeutet, der vor Zelltransformation schützt. Anhand des Modells Drosophila werden die Forschenden des EU-finanzierten Projekts OncoResist die Proliferation von Epithelzellen des Auges nach abnormaler Hippo-Signalgebung untersuchen. Der Schwerpunkt dieser Arbeit liegt dabei auf Veränderungen der nuklearen Landschaft wie der Chromatinzugänglichkeit und der Rolle von langer nicht-kodierender RNA. Die Erforschung dieses Schutzmechanismus wird neue Wege in der Krebsforschung ebnen.
Ziel
Oncogenesis is a complex multi-step process that involves the combination of genetic and epigenetic alterations for normal cells to turn into cancerous cells. Remarkably, while some strong oncogenic signals can promote transformation in some cell states, they are completely innocuous to other cell types.
My aim is to study this poorly understood phenomena, being my current hypothesis, that intrinsic cell state transformation protection mechanisms are likely to exist.
I will use the Drosophila eye primordium epithelium, as I have previously observed that whereas committed epithelial cells over-proliferate in response to abnormal Hippo signalling, undifferentiated progenitor cells are resistant to this transformation.
To molecularly define such resilience to oncogenesis, the gene expression and chromatin accessibility profile of these two cell populations were compared. My preliminary results showed that the chromatin accessibility profile differed significantly and suggested that some pioneer transcription factors primed cells to respond to Hippo signalling. Moreover, I observed that non-coding RNAs were differentially expressed between these two cell populations. In particular, some long non-coding RNAs (lncRNAs), a class of molecules that were recently described as regulators of oncogenic signals, were particularly enriched in the progenitor cell population.
More specifically, I aim to address two main questions:
1 - Is the nuclear landscape (chromatin accessibility and TFs combinations) important to control the response to high oncogenic signals? And how much of this response relies on the activity of pioneer transcription factors?
2 - What is the role of the lncRNAs in controlling cellular response to Hippo signalling?
A better understanding of the intrinsic cell state protective mechanisms against transformation is likely to open important new therapeutic approaches for cancer.
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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordinator
3810-193 Aveiro
Portugal