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Contenu archivé le 2024-04-30

Development of new multisensing biosensors for the detection of bioavailable heavy metals in solid matrices.

Objectif



The following research tasks will be carried out during the project:
1) Design of regulatory cascades and funnels for the simultaneous and specific detection of metal ions.
2) Development of bifunctional reporter systems.
3) Cloning of metal responsive DNA sequences in specialised vectors and expression into A. eutrophus.
4) Overexpression, purification, characterisation of specific and non-specific metal binding proteins.
5) Construction and properties of one gene-based (multisensing) and one protein-based prototype.
6) Testing and evaluation of the biosensors properties on chemically pre-analyzed solid wastes (soils, flyashes, sludges) in lab and field conditions.
7) Comparison of the chemical data and the data generated by the biosensor devices. Inter-laboratory tests.

Appel à propositions

Data not available

Régime de financement

CSC - Cost-sharing contracts

Coordinateur

VITO - Vlaamse Instelling voor Technologisch Onderzoek NV
Contribution de l’UE
Aucune donnée
Adresse
200,Boeretang
2400 Mol
Belgique

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Coût total
Aucune donnée

Participants (5)