Molecular structure and activity of a ribosome inactivation protein ML1 from the mistletoe plant (Viscum album): development of a cDNA expression system for ML1 and structure modelling for molecular replacement

Ziel

An efficient expression system in E. coli for the production of ML1 protein for use in maintaining a future supply of crystals for analysis of the native protein and derivatives, designed to probe the enzyme (A-chain) and lectin (B-chain) activities of the protein, will be designed. This will also enable engineered mutants to be designed and produced for exploitation and development of therapeutic drugs.

The best possible simulated structure models of the A- and B-chains, based on the structure of the homologous protein ricin and known amino acid sequence data, using state-of-the-art software will be developed. These models will provide useful preliminary data on both the enzyme active site of the A-chain and the sugar binding sites of the B-chain and will also enable the hydrophobic regions of the molecule to be mapped. The models will be invaluable for molecular replacement crystal structure determination of the native protein.

Programm/Programme

IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993-

Thema/Themen

42 - Physical Chemical Biology

Aufforderung zur Vorschlagseinreichung

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Finanzierungsplan

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Koordinator

Birkbeck College

EU-Beitrag

Keine Daten

Adresse

Malet Street
WC1E 7HX London
Vereinigtes Königreich

Gesamtkosten

Keine Daten

Beteiligte (1)

Institute of Genetics and Selection of Industrial Microorganisms

Russland

EU-Beitrag

Keine Daten

Ziel

Programm/Programme

Thema/Themen

Aufforderung zur Vorschlagseinreichung

Finanzierungsplan

Koordinator

Beteiligte (1)

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