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Ultrastructure and immunocytochemistry of the cytoskeleton of spermatozoa, eggs, and fertilization in selected invertebrate species for the understanding of phylogeny

Exploitable results

The main results concern the Platyhelminthes, Mollusca and Annelida. a) Platyhelminthes. A better understanding of the sperm structure and spermiogenesis of the Acoel Platyhelminthes, a group previously poorly known from the spermatological point of view, allowed a new interpretation of the phylogenetic relationships of the Paratomellidae. With immunocytochemical methods, it was found that the posttranslational modifications of tubulin (acetylation, glutamylation, glycylation) are different, within a single sperm cell, in the microtubules of the axonemes and in the cytoplasmic microtubules (cortical and axial). The central element of the 9+"1" axoneme is not made up of tubulin. b) Mollusca. The spermatozoa of species from different families of Bivalvia Mytiloidea, Teredinidea and Cardioidea were studied and found to have taxon-specific characters. In Chitons, a parallel evolution of sperm and eggs was discovered, suggesting an evolution of fertilization in the group, and gamete structure was found to be specific for each family. c) Annelida. New models of branchiobdellid spermatozoa from the Far East have helped the understanding of the evolution within the group, in particular underlining a possible origin of the gorup in the Far East, and its relationships with the family Lumbruculidae. d) Echinodermata. Spermatozoa and eggs of different species of sea urchins were studied and were found to belong to the same type. e) Nematoda. Comparative results were obtained, particularly concerning tubulin and other cytoskeletal proteins. The four participant teams agree that the project has provided unique opportunity for developing advanced research and international East-West contacts.

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