Cel
Lymphokine-activated killer (LAK) cells have high anti-tumour activity against autologous and allogenic tumour cells. This cell mediated cytotoxicity is due both to secreted and to membrane-associated cytotoxic proteins. The tumour necrosis factor, lymphotoxin and gamma-interferon, contribute to the cytotoxicity of LAK cells but other cytotoxic factors from LAK cells have not been characterised.
The cytotoxic proteins from LAK cells will be purified in order to determine their partial amino acid sequence and then to clone their genes. Primary structure, immunoreactivity and biological activity of the purified proteins (induction of necrotic or apoptotic pathways of target cells death, specificity to different target cells, etc.) will be compared with the tumour necrosis factor. The results obtained will be valuable for use in continuing research to elucidate the molecular mechanism of anti-tumour activity of cytotoxic lymphocytes and may find applicability in the development of treatment of tumour diseases.
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34012 Trieste
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