Obiettivo This proposal aims at identifying new biomarkers to improve the current approach for assessing the androgen disruption activity of chemicals (type flu amide) on testicular development and function. The objectives are(I) to use a DNA micro array approach to identify among thousands of testicular genes those whose expression is altered in fatally flu amide treated rats and(II) to delineate the potential role of the proteins encoded by these altered genes in the activities of germ cells, Sirloin and Lading cells. The project is divided in 5 work packages including that concerning the project coordination. The WP2 (DNA micro array preparation) and WP3 (tabular tissues from flu amide treated rats) are performed by the industrial partner. Once the altered genes are identified in WP2 and WP3, experiments based on the in vivo and in vitro expression of these genes and transfixion of these genes in testicular cell lines will delineate their potential role in germ cell (Spa, Sterol cell (WP5) and Lydia cell (WP6) specific activities. From these experiments, we expect to (I) identify key genes involved in testicular path physiology related to an endocrine disruption activity and (ii) provide through these relevant genes new fine biomarkers to assess the ant androgen potential of chemicals. Campo scientifico natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicinephysiology Programma(i) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Argomento(i) 1.1.1.-4. - Key action Environment and Health Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Contributo UE Nessun dato Indirizzo ADR 5 - Rhône-Alpes, Auvergne, Bourg. Centre Hos 69495 PIERRE-BENITE Francia Mostra sulla mappa Costo totale Nessun dato Partecipanti (3) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto BAYER CROPSCIENCE SA Francia Contributo UE Nessun dato Indirizzo Rue Dostoievski, 355 06903 SOPHIA ANTIPOLIS Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF TURKU Finlandia Contributo UE Nessun dato Indirizzo Lemminkaesenkatu, 2 20520 TURKU / ABO Mostra sulla mappa Costo totale Nessun dato UTRECHT UNIVERSITY Paesi Bassi Contributo UE Nessun dato Indirizzo Sorbonnelaan 16 3584 CA UTRECHT Mostra sulla mappa Costo totale Nessun dato