Obiettivo
Accumulation of damaged proteins is a hallmark of ageing. Protease is the main proteolysis system in charge of damaged protein degradation within the cell. Recent data on human keratinocytes and dermal fibroblasts have shown that protease alterations may be responsible for the age-related drop of quality in cellular proteins. Our project is a concerted attempt to understand this phenomenon, and to find appropriate handles to keep it under control. We will analyse the expression of genes coding for protease subunits and proteolysis activity. We will use phage-display and antibodies to identify age-associated modifications in cellular proteins. We will construct human cellular model systems to investigate the role of protease in senescence and apoptosis. Finally, we will develop a test to screen substances for their potential effects on age-related protease alterations
Campo scientifico
Invito a presentare proposte
Data not availableMeccanismo di finanziamento
CSC - Cost-sharing contractsCoordinatore
PARIS
Francia