Obiettivo The mitochondria theory of ageing proposes that the accumulation of somatic mutations in mitochondria DNA during life results in a progressive decline in energetic function and eventual cellular and organism senescence. We propose to test this idea directly, by engineering and expressing a version of the mitochondria DNA polymerise POLG that lacks proofreading exonuclease activity, and hence results in the rapid accumulation of medina mutations. This medina matador will be created and tested in parallel in four model systems: yeast, the mouse, cultured human cells and the fruit fly Drosophilae. The phenotypic consequences of an enhanced rate of accumulation of medina mutations will be investigated via studies of cellular bioenergetics function, ageing-related physiological decline and tissue degeneration, alterations in the pattern of gene expression, and overall lifespan. Campo scientifico natural sciencesbiological sciencesgeneticsDNAagricultural sciencesagriculture, forestry, and fisheriesagriculturehorticulturefruit growingnatural sciencesbiological sciencesgeneticsmutation Programma(i) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Argomento(i) 1.1.1.-6. - Key action The Ageing Population and Disabilities Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore UNIVERSITY OF TAMPERE Contributo UE Nessun dato Indirizzo 6,Lenkkeilijänkatu 6 33014 TAMPERE Finlandia Mostra sulla mappa Costo totale Nessun dato Partecipanti (4) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto ASTRA ZENECA AB Svezia Contributo UE Nessun dato Indirizzo Soedertaelje 15185 JAERNA Mostra sulla mappa Costo totale Nessun dato KAROLINSKA INSTITUTE Svezia Contributo UE Nessun dato Indirizzo Karolinska Hospital 171 76 STOCKHOLM Mostra sulla mappa Costo totale Nessun dato UNIVERSIDAD AUTONOMA DE MADRID Spagna Contributo UE Nessun dato Indirizzo Arzobispo Morcillo n2 28029 MADRID Mostra sulla mappa Costo totale Nessun dato UNIVERSITE CATHOLIQUE DE LOUVAIN Belgio Contributo UE Nessun dato Indirizzo Place Croix du Sud 2/19 1348 LOUVAIN-LA-NEUVE Mostra sulla mappa Costo totale Nessun dato
