Objetivo "Plant architecture is largely determined by branching patterns in the shoot and root systems. Shoots of seed plants, such as the model plant Arabidopsis thaliana, branch via lateral bud outgrowth, where branches are patterned along the stem at the base of a leaf. Roots branch laterally from an internal cell layer surrounded by differentiated tissues, at a distance from the root tip. Despite the differences in ontogeny and the divergent evolutionary origin of root and shoot lateral branches, both processes are controlled by the three redundantly-acting AP2-domain containing transcription factors PLETHORA3, 5 and 7 (PLT3,5,7). These proteins control phyllotaxis, the pattern of lateral organs in the shoot, and rhizotaxis, the spacing of lateral roots. Although the phytohormone auxin is known to be intimately involved in both phyllotaxis and rhizotaxis, the PLT3,5,7 module represents the first genetic element to be implicated in control of both processes. Thus, the goal of this proposal is to illuminate the genetic network surrounding PLT3,5,7 gene activity in both the shoot and root contexts. Early results suggest that auxin lies both upstream and downstream of PLT action, pointing to a positive feedback loop which could be at the heart of a pattern generating system. To investigate this network further, genes surrounding PLT action will be identified using genetic, bioinformatic and biochemical strategies. Physical and genetic interactions will place these genes in a genetic hierarchy. Dynamic expression of these genes in living shoots and roots will be monitored using real-time confocal microscopy, and changes in the network dynamics will be observed in plt mutants and in auxin manipulation experiments. The results from this project will illuminate the similarities and differences between these two PLT-bases patterning pathways and will be of interest to developmental, computational and evolutionary biologists alike." Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesevolutionary biologynatural sciencesphysical sciencesopticsmicroscopyconfocal microscopy Programa(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) FP7-PEOPLE-2010-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Convocatoria de propuestas FP7-PEOPLE-2010-IEF Consulte otros proyectos de esta convocatoria Régimen de financiación MC-IEF - Intra-European Fellowships (IEF) Coordinador WAGENINGEN UNIVERSITY Aportación de la UE € 129 416,56 Dirección DROEVENDAALSESTEEG 4 6708 PB Wageningen Países Bajos Ver en el mapa Región Oost-Nederland Gelderland Veluwe Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Peter Van Beem Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Participantes (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo UNIVERSITEIT UTRECHT La participación finalizó Países Bajos Aportación de la UE € 107 833,24 Dirección HEIDELBERGLAAN 8 3584 CS Utrecht Ver en el mapa Región West-Nederland Utrecht Utrecht Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Astrid Haijma (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos