VIROMAProject reference: 612453
Funded under :
Design of a versatile and fast colloidal sensor based on virus modified particles
Total cost:EUR 1 145 078,7
EU contribution:EUR 1 145 078,7
Call for proposal:FP7-PEOPLE-2013-IAPPSee other projects for this call
Funding scheme:MC-IAPP - Industry-Academia Partnerships and Pathways (IAPP)
"Viroma project aims to fabricate multiplexed bead-based arrays as sensing devices on the basis of virus capsid and virosome Layer by Layer-assisted assembly on colloidal carriers. The virosome based platform technology will be suitable for the detection of several analytes ranging from comparatively small molecules, of the size of dioxins to larger biomolecules such as small proteins. Such systems are fast and sensitive, and require due to multiplexing and single particle based fluorometric read-outs only a very small amount of sample for analysis and is, suitable for high throughput analysis. The uniqueness of the approach developed in VIROMA is that the virus particles will retain their specific recognition properties provided by the virus capsides and transfer this specificity to the colloidal carrier. Moreover, the possibility of assembling different virus nanoparticles and virosomes on top of the colloids will result in particles with multiple recognition capabilities, going a step ahead nature.
Depending on the analytes size two different detection mechanisms will be employed. If the analyte is a larger peptide or protein, a sandwich immunoassay can be employed with the capture antibody integrated into the virosome and transferred to the bead by means of virosome-membrane fusion. The fluorescence will be recorded with a flowcytometry using a colloidal dispersion of the beads or a laser scanning microscope if the beads are immobilized on a chip. As an example, we will assemble beads capable of detecting troponin, a standard marker for myocardial infarction. For small molecule s specific receptor molecules will be designed for a competitive assay.
The project involves a synergic collaboration between two academic institutions: CIC biomaGUNE and the University of Leipzig with the company SURFLAY."
EU contribution: EUR 376 437,38
PASEO MIRAMON 182, PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C
20009 SAN SEBASTIAN
Tel.: +34 943005300
EU contribution: EUR 541 272,3
MAX PLANCK STRASSE 3
Tel.: +49 3063921764
Fax: +49 30 63921767
EU contribution: EUR 227 369,02
Tel.: +49 341 9715704