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Design of a versatile and fast colloidal sensor based on virus modified particles

Objective

Viroma project aims to fabricate multiplexed bead-based arrays as sensing devices on the basis of virus capsid and virosome Layer by Layer-assisted assembly on colloidal carriers. The virosome based platform technology will be suitable for the detection of several analytes ranging from comparatively small molecules, of the size of dioxins to larger biomolecules such as small proteins. Such systems are fast and sensitive, and require due to multiplexing and single particle based fluorometric read-outs only a very small amount of sample for analysis and is, suitable for high throughput analysis. The uniqueness of the approach developed in VIROMA is that the virus particles will retain their specific recognition properties provided by the virus capsides and transfer this specificity to the colloidal carrier. Moreover, the possibility of assembling different virus nanoparticles and virosomes on top of the colloids will result in particles with multiple recognition capabilities, going a step ahead nature.

Depending on the analytes size two different detection mechanisms will be employed. If the analyte is a larger peptide or protein, a sandwich immunoassay can be employed with the capture antibody integrated into the virosome and transferred to the bead by means of virosome-membrane fusion. The fluorescence will be recorded with a flowcytometry using a colloidal dispersion of the beads or a laser scanning microscope if the beads are immobilized on a chip. As an example, we will assemble beads capable of detecting troponin, a standard marker for myocardial infarction. For small molecule s specific receptor molecules will be designed for a competitive assay.
The project involves a synergic collaboration between two academic institutions: CIC biomaGUNE and the University of Leipzig with the company SURFLAY.

Call for proposal

FP7-PEOPLE-2013-IAPP
See other projects for this call

Coordinator

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Address
Paseo Miramon 182, Parque Tecnologico De San Sebastian Edificio Empresarial C
20009 San Sebastian
Spain
Activity type
Research Organisations
EU contribution
€ 376 437,37
Administrative Contact
Alfonso Egaña (Mr.)

Participants (2)

Surflay Nanotec GmbH
Germany
EU contribution
€ 541 272,32
Address
Max Planck Strasse 3
12489 Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Claudia Aldenhoven (Dr.)
UNIVERSITAET LEIPZIG
Germany
EU contribution
€ 227 369,01
Address
Ritterstrasse 26
04109 Leipzig
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Edwin Donath (Prof.)