Obiettivo Immune cells constantly receive signalling inputs such as pathogen-emitted molecules, use gene regulatory pathways to process these signals, and generate outputs by secreting signalling molecules like cytokines. Characterizing the input-output relationship of a biological system helps understanding its regulatory mechanisms, and allows building models to predict how the system will operate in complex physiological scenarios, such as population tissue response to infection. A major obstacle in this endeavor has been the so-called “biological noise”, or significant variability in measured molecular parameters between cells. Such variability makes time-dependent single-cell analysis crucial to understand how biological systems operate. Development of new analytical tools with improved functionality, accuracy, and throughput is needed to realize the full potential of single-cell analysis. We propose to develop automated, high-throughput, Optofluidic single-cell analysis systems with unprecedented capabilities, and to use them in understanding how immune cells organize in tissue during response to infection. Microfluidic membrane-valves, nanodroplets, optics, and automation will be integrated to achieve an unparalleled degree of control over single immune cells. Multi-functional lab-on-chip devices will simultaneously measure: a) The activity of immune regulatory proteins such as NF-κB, and b) Inflammatory cytokines secreted from single immune cells in a time-dependent manner, under precisely defined biochemical inputs. Characterizing macrophage cytokine secretion dynamics under combinatorial regiments of bacterial and apoptotic-cell signals will allow dissecting the signalling mechanism responsible from the resolution of inflammation. We will identify the role of the NF-κB pathway in regulation of cytokine dynamics. We will use our data to develop a computer model of tissue-level immune response to pathogens through the NF-κB pathway and cytokine signaling. Campo scientifico engineering and technologyother engineering and technologiesmicrotechnologylab on a chipnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinssocial sciencessociologyindustrial relationsautomationmedical and health sciencesbasic medicineimmunologynatural sciencesphysical sciencesoptics Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-SG-PE4 - ERC Starting Grant - Physical and Analytical Chemical sciences Invito a presentare proposte ERC-2013-StG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-SG - ERC Starting Grant Istituzione ospitante EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH Contributo UE € 1 499 165,00 Indirizzo Raemistrasse 101 8092 Zuerich Svizzera Mostra sulla mappa Regione Schweiz/Suisse/Svizzera Zürich Zürich Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Savas Tay (Prof.) Ricercatore principale Savas Tay (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH Svizzera Contributo UE € 1 499 165,00 Indirizzo Raemistrasse 101 8092 Zuerich Mostra sulla mappa Regione Schweiz/Suisse/Svizzera Zürich Zürich Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Savas Tay (Prof.) Ricercatore principale Savas Tay (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
