Objective The work aims at the development and construction of advanced laser desorption ionization mass spectrometry instrumentation with special emphasis on the analytical demands of molecular biology research and biotechnology. The instrumental set-up will be modular with a basic configuration for routine molecular mass screening of biopolymers and mixtures thereof by matrix-assisted laser desorption ionization (MALDI) with high accuracy and sensitivity. Upgrading comprises an ion reflectron for improved mass resolution and mass accuracy emphasizing the capability to perform structural analysis of the analytes by analysis of metastable fragment ions (PSD : post-source-decay analysis) e.g. for peptide sequencing and glycoconjugate investigation. Upgrading furthermore includes the implementation of appropriate sample stages in order to couple MALDI-MS to routine liquid separation techniques, especially to (micro-)HPLC and capillary electrophoresis. Within the project, procedural protocols are to be developed both to improve the current performance of the MALDI technique with respect to reproducibility of preparations including tolerance against contaminants and shot-to-shot fluctuations and to adapt these protocols to the requirements of samples eluted from liquid chromatography. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesnatural scienceschemical scienceselectrochemistryelectrophoresisnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesphysical sciencesopticslaser physicsnatural sciencesbiological sciencesmolecular biology Programme(s) FP3-MAT - Specific research and technological development programme (EEC) in the field of measurements and testing, 1990-1994 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator WESTFAELISCHE WILHELMS-UNIVERSITAET MUENSTER EU contribution No data Address RONTGENSTRASSE 9-13 48149 MUENSTER Germany See on map Total cost No data Participants (3) Sort alphabetically Sort by EU Contribution Expand all Collapse all HEINRICH-HEINE-UNIVERSITAET DUESSELDORF Germany EU contribution No data Address Universitaetsstr. 1 40225 DUESSELDORF See on map Total cost No data Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek Netherlands EU contribution No data Address 48,Utrechtseweg 3700 AJ Zeist See on map Total cost No data TECHNICAL UNIVERSITY OF CRETE Greece EU contribution No data Address Eleftheriou Venizelou 34 72132 CHANIA See on map Total cost No data
