Objetivo Superior enantioselectivity is an important driving force for the proliferation of enzymes in industrial biotransformations. However, on expanding the scope of enzymatic conversions to organic media substantial deviations from the intrinsic selectivity in aqueous solution have been observed. Ambiguous enantioselective behaviour of enzymes in organic solvents frustrates their use as customised catalysts in industrial processes. Since current attempts to establish (semi-) empirical correlations of enzyme enantioselectivity with bulk solvent properties turn out to be of limited value, fundamental studies are required. Main objective of this project is the understanding of medium effects on enzyme enantioselectivity. Our strategy involves the rigorous evaluation of mechanistic, kinetic, and thermodynamic contributions that may affect the enantioselective performance of lipases from Rhizomucor miehei and Candida antarctica in kinetic resolutions and asymmetrizations of chiral and prochiral alcohols and acids in organic media. R. miehei and C. antarctica lipases are versatile catalysts with great industrial potential. Extensive structural information is available for both enzymes. Judicious combination of dynamic and structural knowledge will provide a frame of reference against which molecular modelling techniques can be properly validated. By performing convergent mechanistic, thermodynamic and molecular modelling investigations on a limited set of targets, the participating groups expect to gain insight that will open novel perspectives for the rational design of medium-engineering tools applicable to a broad range of enzyme-catalysed kinetic resolutions of industrial interest. Ámbito científico natural sciencesphysical sciencesthermodynamicsnatural scienceschemical sciencesorganic chemistryalcoholsnatural scienceschemical sciencescatalysisnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP4-BIOTECH 2 - Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998 Tema(s) 0102 - Biochemical engineering components of cell factories Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador Technische Universiteit Delft Aportación de la UE Sin datos Dirección 67,Julianalaan 2628 BC Delft Países Bajos Ver en el mapa Coste total Sin datos Participantes (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Consiglio Nazionale delle Ricerche Italia Aportación de la UE Sin datos Dirección 9,Via Mario Bianco 9 20131 Milano Ver en el mapa Coste total Sin datos ROYAL INSTITUTE OF TECHNOLOGY Suecia Aportación de la UE Sin datos Dirección 34,Roslagstullsbacker, 21 100 44 STOCKHOLM Ver en el mapa Coste total Sin datos
