Objetivo The goal of this project is to study the mechanisms underlying the modulation by second messengers of nicotinic acetylcholine receptor (nAChR)-activated ionic channels in muscle, in neurons of invertebrates (snail) and in vertebrates (sympathetic ganglion of the rat). A combination of the following techniques will be used: electrophysiology (patch-clamp, microelectrode, and intracellular dialysis recording); chemistry (synthesis of new nACh receptor-channel modulators); and fluorescence image analysis (confocal laser scanning microscopy). The following areas will be investigated: the comparative properties of whole-cell AChR-activated currents in mouse myotubes and in sympathetic ganglia neurons; modulation of nAChR by four protein kinase systems (cAMP- and cGMP-dependent phosphorylation; the protein kinase C- and tyrosine kinase-dependent phosphorylation); the modulation of ACh-activated currents in snail neurons by cytoskeleton; the pathways involved in intracellular Ca 2+ ([Ca 2+]i) elevation at AChR-activation and the modulation of AChR channels by [Ca 2+]i.This study will make it possible to elucidate mechanisms of nAChR channels modulation by intracellular phosphorylation and by the state of neuronal cytoskeleton. Programa(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Tema(s) 42 - Physical Chemical Biology Convocatoria de propuestas Data not available Régimen de financiación Data not available Coordinador Institut National de la Santé et de la Recherche Médicale Aportación de la UE Sin datos Dirección Bld. de Port Royal 123 75674 Paris 14 Francia Ver en el mapa Coste total Sin datos Participantes (4) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Institute of Cell Biophysics Rusia Aportación de la UE Sin datos Dirección 142292 Pushchino, Moscow Region Ver en el mapa Coste total Sin datos National Academy of Sciences of Ukraine Ucrania Aportación de la UE Sin datos Dirección 252024 Kiev Ver en el mapa Coste total Sin datos Russian Academy of Sciences Rusia Aportación de la UE Sin datos Dirección 197376 St. Petersburg Ver en el mapa Coste total Sin datos Università degli Studi di Roma La Sapienza Italia Aportación de la UE Sin datos Dirección 00158 Roma Ver en el mapa Coste total Sin datos
