Objetivo To increase successful applications of retrovirus delivery in gene therapy this demonstration project integrates and validates novel but robust technologies:i) Specific genetically stable and safer human diploid cells yield higher virus titers than established, transformed cell lines;ii) Modular master producer cell lines permit predictable expression properties of the therapeutic construct and envelope genes, inserted precisely at defined, optimal loci, conserving expression levels, physiological, genetic stability and safety advantages of the master cells;iii)Medium, cultivation, purification and storage improvements result in maximal cell and tissue infectivity;iv) Safety and efficacy of cells and retroviral vectors will be thoroughly tested and certified, including examination in solid tissue organ systems;v) A final cGMP run will validate and complete the integrated strategy to produce clinically applicable material for trials against dystrophic epidermolysis bullosa. Ámbito científico medical and health sciencesmedical biotechnologygenetic engineeringgene therapynatural sciencesbiological sciencesmicrobiologyvirology Programa(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Tema(s) 1.1.1.-3. - Key action The "Cell factory" Convocatoria de propuestas Data not available Régimen de financiación DEM - Demonstration contracts Coordinador INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLOGICA Aportación de la UE Sin datos Dirección Quinta do Marques 2780-901 OEIRAS Portugal Ver en el mapa Coste total Sin datos Participantes (4) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo GBF - NATIONAL CENTRE FOR BIOTECHNOLOGY Alemania Aportación de la UE Sin datos Dirección Mascheroder Weg 1 38124 BRAUNSCHWEIG Ver en el mapa Coste total Sin datos GENETHON III Francia Aportación de la UE Sin datos Dirección RUE DE L'INTERNATIONALE 1BIS 91002 EVRY Ver en el mapa Coste total Sin datos HENOGEN S.A. Bélgica Aportación de la UE Sin datos Dirección Rue des Professeurs Jaener et Brachet 12 6041 GOSSELIES Ver en el mapa Coste total Sin datos THE HEBREW UNIVERSITY OF JERUSALEM - THE AUTHORITY FOR RESEARCH AND DEVELOPMENT Israel Aportación de la UE Sin datos Dirección Givat Ram Campus, Edmond Safra 91904 JERUSALEM Ver en el mapa Coste total Sin datos
