Objetivo The subject of this study is mRNA expression profiling of differentiating murine hippocampal neurons. A cDNA microarray approach will be used to simultaneously quantify expression levels of about seven thousand of mRNAs at morphologically and functionally defined stages of neuronal differentiation. A culture system will be used in which neurons undergo differentiation, in a well-coordinated manner, to a fully polarised pyramidal phenotype with functional synapses. The objectives of this study are:1. quantitative analysis of expression of a large mRNA set at defined stages of neuronal differentiation2. Identification of genes that are regulated in the differentiation process3. Cluster analysis of genes according to their expression patterns. The results of this project will form a basis for future work; on regulatory mechanisms underlying coordinated expression of genes in differentiating neurons; and on functional relationships of protein products of the developmentally regulated genes. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteins Programa(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Tema(s) 1.1.1.-6. - Key action The Ageing Population and Disabilities Convocatoria de propuestas Data not available Régimen de financiación RGI - Research grants (individual fellowships) Coordinador FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW Aportación de la UE Sin datos Dirección 498BUS1,Herestraat 49 KUL / Campus Gasthuisberg 3000 LOUVAIN / LEUVEN Bélgica Ver en el mapa Coste total Sin datos