Obiettivo The current application concerns the mechanisms of transcription initiation in eukaryotic cells. The postdoc-project will be carried out in Prof. Roger D. Kornberg's laboratory at Stanford University, California.THE SPECIFIC AIM of the project is a molecular understanding of how RNA polymerase II (RNAP II) together with the general transcription factors (GTFs) can recognize promoter DNA and initiate RNA synthesis at a specific genomic location.Reports have shown that RNAP II and TFIIB are solely responsible for this process, but the underlying mechanism is still not known. Recently the structure of a RNAP II/TFIIB cocrystal has been solved in budding yeast. The transcription start site differs however in this yeast compared to fission yeast and most other eukaryotes including human so a general model for the selection process could not be elucidated from this structure.OUR MAIN OBJECTIVES are to do structural analysis of a RNAP II/TFIIB complex in fission yeast with X-ray crystallography and compare it to t he budding yeast structure. We will complement the structural information with mutational analysis of TFIIB in a pure in vitro transcription system derived from fission yeast developed by me as a Ph.D. student.THE OVERALL GOAL with the project is to generate a general molecular model for the transcription start site selection process.My stay will lead to the acquirement of new technological knowledge such as structural analysis of multi-protein complexes with X-ray crystallography that I believe will be a vibrant scientific field in the future.I will also be able to develop a scientific network that will be useful for my future career as a scientist, as well as the European community, after my return to Sweden. Campo scientifico natural sciencesbiological sciencesgeneticsDNAnatural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural sciencesbiological sciencesgeneticsRNA Parole chiave RNA polymerase II Start site selection TFIIB Transcription X-ray crystallography of macromolecular structures Programma(i) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Argomento(i) MOBILITY-2.2 - Marie Curie Outgoing International Fellowships (OIF) Invito a presentare proposte FP6-2002-MOBILITY-6 Vedi altri progetti per questo bando Meccanismo di finanziamento OIF - Marie Curie actions-Outgoing International Fellowships Coordinatore KAROLINSKA INSTITUTET Contributo UE Nessun dato Indirizzo Nobels väg 5 STOCKHOLM Svezia Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto STANFORD UNIVERSITY, SCHOOL OF MEDICINE Stati Uniti Contributo UE Nessun dato Indirizzo Dept. of Structural Biology, 299 Campus Drive West, Fairchild Science Building STANFORD, CALIFORNIA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato
