Objective Cell migration is a key step for the development of inflammatory and metastatic disease. Mechanisms involved in cell migration include alterations in adhesion molecules and chemokine receptor status, activation of signalling molecules and cytoskeletal rearrangement. Cell-based high content screening (HCS) is a revolutionary technology which allows subcellular imaging of temporary cellular events as well as analysis of intracellular events, permitting the study of deeper biological information at the sub-cellular level.Hence, due to its high throughput capacity, this technology is likely to improve the bottlenecks in the process leading towards drug discovery, and the establishment of this technological platform in Europe will have immediate applications to many of the causes of morbidity and mortality within the European Union. The laboratory of Prof. Dermot Kelleher, the coordinator of this proposal, is one of the first academic institutions in Europe to acquire such a technology. In this application we propose to combine the efficiency of inhibitory small inhibitory RNA (siRNA) libraries with the high throughput capacity of cell-based HCS to the study of cell migration in the context of neoplastic diseases and inflammation.This project requests research fellows with expertise in the areas of cell biology/cell migration combined with HCS, IT/bioinformatics, molecular biologists with experience of inhibitory RNA and high throughput transfection technologies and cell biologists with industrial/drug screening experience. This transfer of knowledge to the host institution using siRNA in combination with HCS to study the molecular mechanisms of cell migration will be essential to help establish and develop this novel technology and its use to accelerate the process of drug discovery. Fields of science medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverynatural sciencesbiological sciencescell biologynatural sciencesphysical sciencesopticsmicroscopynatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysisnatural sciencesbiological sciencesgeneticsRNA Keywords Cell shape change Content High Content Screening Inflammation Inflammation Metastasis Cell Metastasis Screening gene change High gene silencing motility Cell shape siRNA silencing siRNA drug targets Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Topic(s) MOBILITY-1.3 - Marie Curie Host Fellowships - Transfer of knowledge (TOK) Call for proposal FP6-2004-MOBILITY-3 See other projects for this call Funding Scheme TOK - Marie Curie actions-Transfer of Knowledge Coordinator THE PROVOST FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN EU contribution No data Address DUBLIN Ireland See on map Links Website Opens in new window Total cost No data Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all CELLOMICS INC United States EU contribution No data Address 100 Technology Drive PITTSBURGH PA See on map Total cost No data BENITEC LTD. United States EU contribution No data Address 2375 Garcia Avenue CALIFORNIA See on map Total cost No data
