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Content archived on 2024-06-16

Study of the molecular mechanisms of cell migration by using high content screening and siRNA technology

Objective

Cell migration is a key step for the development of inflammatory and metastatic disease. Mechanisms involved in cell migration include alterations in adhesion molecules and chemokine receptor status, activation of signalling molecules and cytoskeletal rearrangement. Cell-based high content screening (HCS) is a revolutionary technology which allows subcellular imaging of temporary cellular events as well as analysis of intracellular events, permitting the study of deeper biological information at the sub-cellular level.

Hence, due to its high throughput capacity, this technology is likely to improve the bottlenecks in the process leading towards drug discovery, and the establishment of this technological platform in Europe will have immediate applications to many of the causes of morbidity and mortality within the European Union. The laboratory of Prof. Dermot Kelleher, the coordinator of this proposal, is one of the first academic institutions in Europe to acquire such a technology. In this application we propose to combine the efficiency of inhibitory small inhibitory RNA (siRNA) libraries with the high throughput capacity of cell-based HCS to the study of cell migration in the context of neoplastic diseases and inflammation.

This project requests research fellows with expertise in the areas of cell biology/cell migration combined with HCS, IT/bioinformatics, molecular biologists with experience of inhibitory RNA and high throughput transfection technologies and cell biologists with industrial/drug screening experience. This transfer of knowledge to the host institution using siRNA in combination with HCS to study the molecular mechanisms of cell migration will be essential to help establish and develop this novel technology and its use to accelerate the process of drug discovery.

Fields of science (EuroSciVoc)

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Keywords

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Topic(s)

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Call for proposal

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FP6-2004-MOBILITY-3
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Funding Scheme

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TOK - Marie Curie actions-Transfer of Knowledge

Coordinator

THE PROVOST FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN
EU contribution
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Total cost

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Participants (2)

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