Obiettivo A fundamental but unexplored problem in biology is whether and how enzymes use mechanical strain during their functioning. It is now evident that the knowledge of atomic structures and chemical interactions is not sufficient to understand the intricate mechanisms underlying enzyme specificity and efficiency. Several lines of evidence suggest that mechanical effects play crucial roles in enzyme activity. Therefore we aim to create detailed force maps that reveal how the intramolecular distribution of mechanical strains changes during the enzyme cycle and how these rearrangements drive the enzyme processes. The applicability of current nanotechniques for the investigation of this problem is limited because they do not allow simultaneous measurement of mechanical and enzymatic parameters. Thus we seek to open new avenues of research by developing site-specific sensors and passive or photoinducible molecular springs to measure force-dependent chemical/structural changes with high spatiotemporal resolution in myosin. Since force perturbations occur very rapidly, we are able to combine experimental studies with quasi-realistic in silico simulations to describe the physical background of enzyme function. We expect that our research will yield fundamental insights into the role of intramolecular strains in enzymes and thus greatly aid the design and control of enzyme processes (specificity, activity, regulation). Our studies may also lead to new paradigms in the understanding of motor systems. Campo scientifico engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Parole chiave dynamics enzyme kinase kinetics mechanics mechanism motor protein Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry Invito a presentare proposte ERC-2007-StG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-SG - ERC Starting Grant Istituzione ospitante EOTVOS LORAND TUDOMANYEGYETEM Contributo UE € 750 000,00 Indirizzo EGYETEM TER 1-3 1053 Budapest Ungheria Mostra sulla mappa Regione Közép-Magyarország Budapest Budapest Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Katalin Juhászné Huszty (Dr.) Ricercatore principale András Málnási-Csizmadia (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto EOTVOS LORAND TUDOMANYEGYETEM Ungheria Contributo UE € 750 000,00 Indirizzo EGYETEM TER 1-3 1053 Budapest Mostra sulla mappa Regione Közép-Magyarország Budapest Budapest Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Katalin Juhászné Huszty (Dr.) Ricercatore principale András Málnási-Csizmadia (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
