Objective
Successful and efficient plant breeding depends on rapid recombination of advantageous traits to form new crop varieties. In recent years new breeding techniques have been introduced which rely on transgenic alteration of somatic cells and regeneration into plants with novel properties. The precision and effectiveness of both strategies rely upon homologous recombination (HR). The objective of this proposal is to provide plant breeders with new tools allowing better control over HR in both somatic and meiotic cells. The expected outcomes of the proposed research are efficient gene targeting (GT) technologies for precise engineering of plant genomes and control of rates of meiotic recombination between homologous or homeologous chromosomes in classical breeding. The major components of the HR machinery are common to somatic and meiotic cells, enabling us to address both processes in a synergistic way. HR can be divided into different steps: initiation by formation of a DNA double-strand break (DSB); recognition and invasion of an homologous DNA sequence; resolution of recombination structures. Each stage contains a bottleneck for both GT and meiotic HR that we will address. Work package 1 (WP1) aims at enhancing HR through targeted DSB induction. DSBs will be induced by Zinc-finger nucleases that can be custom-designed for target sequences anywhere in the genome. In WP2, we will test the influence of HR factors affecting homologue invasion and heteroduplex formation, such as RAD51 and its paralogues, the RAD52 homologue, genes that affect cytosine methylation in DNA, and mismatch repair. In WP3 we will concentrate on proteins involved in resolution and crossing-over. WP4 will test combinations of those approaches found in the first three WPs to build optimal strategies for application. Most experiments will be performed in the model plant Arabidopsis and implemented into crops such as tomato and maize to guarantee quick applicability for breeding.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy plant breeding
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-KBBE-2008-2B
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
76131 Karlsruhe
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.