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Recombination: an old and new tool for plant breeding

Objective

Successful and efficient plant breeding depends on rapid recombination of advantageous traits to form new crop varieties. In recent years new breeding techniques have been introduced which rely on transgenic alteration of somatic cells and regeneration into plants with novel properties. The precision and effectiveness of both strategies rely upon homologous recombination (HR). The objective of this proposal is to provide plant breeders with new tools allowing better control over HR in both somatic and meiotic cells. The expected outcomes of the proposed research are efficient gene targeting (GT) technologies for precise engineering of plant genomes and control of rates of meiotic recombination between homologous or homeologous chromosomes in classical breeding. The major components of the HR machinery are common to somatic and meiotic cells, enabling us to address both processes in a synergistic way. HR can be divided into different steps: initiation by formation of a DNA double-strand break (DSB); recognition and invasion of an homologous DNA sequence; resolution of recombination structures. Each stage contains a bottleneck for both GT and meiotic HR that we will address. Work package 1 (WP1) aims at enhancing HR through targeted DSB induction. DSBs will be induced by Zinc-finger nucleases that can be custom-designed for target sequences anywhere in the genome. In WP2, we will test the influence of HR factors affecting homologue invasion and heteroduplex formation, such as RAD51 and its paralogues, the RAD52 homologue, genes that affect cytosine methylation in DNA, and mismatch repair. In WP3 we will concentrate on proteins involved in resolution and crossing-over. WP4 will test combinations of those approaches found in the first three WPs to build optimal strategies for application. Most experiments will be performed in the model plant Arabidopsis and implemented into crops such as tomato and maize to guarantee quick applicability for breeding.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/chromosome
  • /agricultural sciences/agriculture, forestry, and fisheries/agriculture/plant breeding
  • /natural sciences/biological sciences/genetics and heredity/heredity
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /natural sciences/biological sciences/genetics and heredity/genome
  • /agricultural sciences/agriculture, forestry, and fisheries/agriculture/plant breeding/crops

Call for proposal

FP7-KBBE-2008-2B
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

KARLSRUHER INSTITUT FUER TECHNOLOGIE
Address
Kaiserstrasse 12
76131 Karlsruhe
Germany
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 451 460
Administrative Contact
Holger Puchta (Dr.)

Participants (7)

WEIZMANN INSTITUTE OF SCIENCE
Israel
EU contribution
€ 405 220
Address
Herzl Street 234
7610001 Rehovot
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Talia Tzahor (Ms.)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 404 169
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
Administrative Contact
Sandrine Magnetto (Dr.)
UNIVERSITE DE GENEVE
Switzerland
EU contribution
€ 396 708
Address
Rue Du General Dufour 24
1211 Geneve
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jerzy Paszkowski (Prof.)
UNIVERSITEIT LEIDEN
Netherlands
EU contribution
€ 400 302
Address
Rapenburg 70
2311 EZ Leiden
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ton Brouwer (Mr.)
KEYGENE NV
Netherlands
EU contribution
€ 389 308
Address
Agro Business Park 90
6700AE Wageningen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Michiel De Both (Dr.)
BIOGEMMA
France
EU contribution
€ 369 500
Address
Rue E. Colonne 1
75001 Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Catherine Guichard (Dr.)
OSM-DAN LTD
Israel
EU contribution
€ 180 480
Address
19 Pro Neeman Yuval St
7608665 Rehovot
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Pnina Dan (Dr.)