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Content archived on 2024-06-18

Health Impact of Engineered Metal and Metal Oxide Nanoparticles: Response, Bioimaging and Distribution at Cellular and Body Level

Objective

Metal oxide and metal NPs are particularly dangerous for two reasons: their special catalytic activity coming from the properties of their nanointerface may interfere with numerous intracellular biochemical processes and the decomposition of NPs and the ion leakage could heavily interfere with the intracellular free metal ion homeostasis, which is essential for cell metabolism. A very specific problem is the difficulty of localizing and quantifying them in cells. Obtaining dose effect relationships is not simple, because of the unknown amount of material present in affected cells. The following main points will be addressed in this proposal:1) Design and synthesis of metal oxide and metal NPs, which can be traced by SPECT, PET, and fluorescence techniques and the appropriate characterization of these NPs.2) Application of label-free techniques, such as IBM and EM to ensure that the radioactive and fluorescent constituents do not modify the cytological and organismic response by themselves.3) Characterization of the uptake, distribution kinetics and NP release at the level of the organism.4) Study of the interaction of NPs with plasma components forming complexes with NPs and the assessment of their possible impact on the uptake compared with that of bare or capped particles.5) Quantification and localization of metallic NPs in immune competent cells is a key task for the establishment of proper dose-response correlations. A technique applicable with living cells as ultimate control will be IBM, capable of detecting single metal NPs in cells at different depths.6) Development of sophisticated cell physiological approaches focusing on the determination of oxidative activity, cytokine production and adaptive processes concerning signalling pathways beyond standard vitality tests. The research project will indicate toxic levels of various NPs and sub-toxic effects will be investigated by analysing the signalling response of immune cells

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-NMP-2008-SMALL-2
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

CP-FP - Small or medium-scale focused research project

Coordinator

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
EU contribution
€ 506 880,33
Address
PASEO MIRAMON 182, PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C
20009 San Sebastian
Spain

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Region
Noreste País Vasco Gipuzkoa
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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Participants (8)

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