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Health Impact of Engineered Metal and Metal Oxide Nanoparticles: Response, Bioimaging and Distribution at Cellular and Body Level

Objective

Metal oxide and metal NPs are particularly dangerous for two reasons: their special catalytic activity coming from the properties of their nanointerface may interfere with numerous intracellular biochemical processes and the decomposition of NPs and the ion leakage could heavily interfere with the intracellular free metal ion homeostasis, which is essential for cell metabolism. A very specific problem is the difficulty of localizing and quantifying them in cells. Obtaining dose effect relationships is not simple, because of the unknown amount of material present in affected cells. The following main points will be addressed in this proposal:1) Design and synthesis of metal oxide and metal NPs, which can be traced by SPECT, PET, and fluorescence techniques and the appropriate characterization of these NPs.2) Application of label-free techniques, such as IBM and EM to ensure that the radioactive and fluorescent constituents do not modify the cytological and organismic response by themselves.3) Characterization of the uptake, distribution kinetics and NP release at the level of the organism.4) Study of the interaction of NPs with plasma components forming complexes with NPs and the assessment of their possible impact on the uptake compared with that of bare or capped particles.5) Quantification and localization of metallic NPs in immune competent cells is a key task for the establishment of proper dose-response correlations. A technique applicable with living cells as ultimate control will be IBM, capable of detecting single metal NPs in cells at different depths.6) Development of sophisticated cell physiological approaches focusing on the determination of oxidative activity, cytokine production and adaptive processes concerning signalling pathways beyond standard vitality tests. The research project will indicate toxic levels of various NPs and sub-toxic effects will be investigated by analysing the signalling response of immune cells

Field of science

  • /medical and health sciences/basic medicine/physiology/homeostasis
  • /natural sciences/biological sciences/cell biology/cell metabolism
  • /natural sciences/physical sciences/theoretical physics/particles

Call for proposal

FP7-NMP-2008-SMALL-2
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project
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Coordinator

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Address
Paseo Miramon 182, Parque Tecnologico De San Sebastian Edificio Empresarial C
20009 San Sebastian
Spain
Activity type
Research Organisations
EU contribution
€ 506 880,33
Administrative Contact
Sergio Enrique Moya (Dr.)

Participants (8)

UNIVERSIDAD DE VIGO
Spain
EU contribution
€ 339 678,02
Address
Lg Campus Lagoas Marcosende
36310 Vigo Pontevedra
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Africa González Fernández (Dr.)
UNIVERSITAET LEIPZIG
Germany
EU contribution
€ 511 730,40
Address
Ritterstrasse 26
04109 Leipzig
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Edwin Donath (Prof.)
EDUCACAO E ENSINO SUPERIOR DO ALTO AVE S.A.

Participation ended

Portugal
Address
Quinta De Matos-geraz Do Minho
4830-316 Povoa De Lanhoso
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Marta Marques (Ms.)
CENTRO DE INVESTIGACION EN QUIMICA APLICADA
Mexico
EU contribution
€ 110 499,38
Address
Bd Enrique Reyna Hermosillo 140
25253 Saltillo
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ronald F. Ziolo (Prof.)
Zhejiang University
China
EU contribution
€ 98 141,27
Address
Zhe Da Road 38
310027 Hangzhou
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Changyou Gao (Prof.)
PLASMACHEM PRODUKTIONS- UND HANDEL GMBH
Germany
EU contribution
€ 241 200
Address
Schwarzschildstrasse 10
12489 Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Alexei Antipov (Dr.)
DET NATIONALE FORSKNINGSCENTER FORARBEJDSMILJO
Denmark
EU contribution
€ 330 108
Address
Lerso Parkalle 105
2100 Kobenhavn
Activity type
Research Organisations
Administrative Contact
Soeren Thor Larsen (Dr.)
TYOTERVEYSLAITOS
Finland
EU contribution
€ 159 099,60
Address
Topeliuksenkatu 41 B
00250 Helsinki
Activity type
Research Organisations
Administrative Contact
Kai Savolainen (Prof.)