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Content archived on 2024-06-18

Application of massive parallel sequencing to microRNA profiling of cellular and molecular response to HIV-1 and HIV-2 infection and to modulation of integration

Objective

miRNAs are key regulators of cell function and pathogen infection. The identification of miRNAs involved inHIV infection promises to offer unique insights into disease mechanisms and to help identify novel targets for therapy. However, the ability to perform an unbiased characterization of miRNA expression has only recently become possible through the use of massive parallel sequencing. This proposal aims to generate the first whole-genome map of miRNA expression during HIV-1 and HIV-2 infection in primary lymphocytes using these novel sequencing techniques. Furthermore, we will identify miRNAs associated with key steps of infection by combining sequencing with the use of drug inhibitors that block viral DNA integration. We expect to identify miRNAs required for the progression of viral infection, with potential for future use in anti-HIV therapy. Pathways associated with differential miRNA expression between HIV-1 and HIV-2 will provide insights into the mildness of HIV-2 infection, providing clues for new strategies against HIV-1.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

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FP7-PEOPLE-2009-RG
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-ERG - European Re-integration Grants (ERG)

Coordinator

FUNDACAO DA FACULDADE DE CIENCIAS DA UNIVERSIDADE DE LISBOA FP
EU contribution
€ 45 000,00
Address
CAMPO GRANDE EDIFICIO C1 PISO 3
1749 016 Lisboa
Portugal

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Region
Continente Área Metropolitana de Lisboa Área Metropolitana de Lisboa
Activity type
Research Organisations
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Total cost

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