Objective
The aim of this project is to analyze dynamics of chromatin-related proteins in living cells after induced DNA damage.
Cells have developed sophisticated mechanisms to overcome damage to DNA resulting in double-strand breaks (DSBs) induced by toxic agents of our environment. Improper repair of DSBs can lead to the development of cancer. Once a cancer has developed, radiation and chemotherapy are used to damage DNA in order to kill the tumor cells. Thus, the information of how cells respond to DNA damage is critical for understanding both the development of cancer and its therapy. We want to contribute to this knowledge by the elucidation of the processes changing the chromatin structure in the DSB vicinity. Chromatin changes represent the fundamental response of the cell to DSB induction that should allow the access of repair proteins to DNA for efficient signaling and repair. It should be found how the chromatin structure is modified, what is the difference in chromatin dynamics in open and compact chromatin compartments and how are all these processes coordinated in time to allow accumulation of repair proteins for full restoration of genome integrity.
The synergy of Czech expertise and the experience of Polish and Russian partners in these areas is a perfect match that will allow further advancements of science. Integration of the newest equipments and technologies together: 3D Microscopy of living cells, FISH technique, immunohistochemistry, RT-PCR and Western blotting, ChIP-PCR, ChIP-on-chips, FRAP, FLIP FRET techniques (Czech and Polish partners) and Scanning Flow Cytometry, Fluorescence Microscopy (Russian partners) should give the response to reach the aim of this project.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
- medical and health sciences clinical medicine oncology
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-IRSES
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MC-IRSES - International research staff exchange scheme (IRSES)
Coordinator
612 65 Brno
Czechia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.