Obiettivo The aim of this project is to analyze dynamics of chromatin-related proteins in living cells after induced DNA damage.Cells have developed sophisticated mechanisms to overcome damage to DNA resulting in double-strand breaks (DSBs) induced by toxic agents of our environment. Improper repair of DSBs can lead to the development of cancer. Once a cancer has developed, radiation and chemotherapy are used to damage DNA in order to kill the tumor cells. Thus, the information of how cells respond to DNA damage is critical for understanding both the development of cancer and its therapy. We want to contribute to this knowledge by the elucidation of the processes changing the chromatin structure in the DSB vicinity. Chromatin changes represent the fundamental response of the cell to DSB induction that should allow the access of repair proteins to DNA for efficient signaling and repair. It should be found how the chromatin structure is modified, what is the difference in chromatin dynamics in open and compact chromatin compartments and how are all these processes coordinated in time to allow accumulation of repair proteins for full restoration of genome integrity.The synergy of Czech expertise and the experience of Polish and Russian partners in these areas is a perfect match that will allow further advancements of science. Integration of the newest equipments and technologies together: 3D Microscopy of living cells, FISH technique, immunohistochemistry, RT-PCR and Western blotting, ChIP-PCR, ChIP-on-chips, FRAP, FLIP FRET techniques (Czech and Polish partners) and Scanning Flow Cytometry, Fluorescence Microscopy (Russian partners) should give the response to reach the aim of this project. Campo scientifico natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsmicroscopymedical and health sciencesclinical medicineoncologynatural sciencesbiological sciencesgeneticsgenomes Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) FP7-PEOPLE-2010-IRSES - Marie Curie Action "International Research Staff Exchange Scheme" Invito a presentare proposte FP7-PEOPLE-2010-IRSES Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IRSES - International research staff exchange scheme (IRSES) Coordinatore BIOFYZIKALNI USTAV AKADEMIE VED CESKE REPUBLIKY Contributo UE € 70 300,00 Indirizzo Kralovopolska 135 CZ-61265 Brno Cechia Mostra sulla mappa Regione Česko Jihovýchod Jihomoravský kraj Tipo di attività Research Organisations Contatto amministrativo Eva Bartova (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Partecipanti (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto Jagiellonian University in Krakow Polonia Contributo UE € 62 700,00 Indirizzo Ul. Golebia 24 31007 KRAKOW Mostra sulla mappa Regione Makroregion centralny Łódzkie Miasto Łódź Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Jerzy Dobrucki (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
