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A binary sensor with single-molecule digit to discriminate biofluids enclosing zero or at least one biomarker

Project description

A binary platform for biomarker detection

Early diagnosis of many diseases improves clinical outcomes as it offers the opportunity to provide care at the earliest possible stage. To be able to implement such a public health strategy in all settings, there is a need for disease-specific biomarkers that can be monitored non-invasively. The mission of the EU-funded NoOne project is to develop a bioelectronic sensor that can measure both genomic and protein-based biomarkers in various biological samples. The NoOne platform will offer results in a binary form, recording samples with at least one positive biomarker, and assist the detection of diseases such as cancer and bacterial infections in a reliable, fast and cost-effective way.

Objective

Biomarkers are measurable indicators of a particular disease state of an organism. There has been an increasing demand for diagnostic markers, enabling reliable and non-invasive screening of peripheral biofluids. It is also established that a universal screening against life-threatening diseases, such as cancer, or infectious disease outbreaks, can be accomplished only by combining genomic and protein marker-based tests. The NoOne project aims at conceiving, engineering, fabricating and validating a ground-breaking platform based on a single-molecule binary bioelectronic sensor, capable to reliably discriminate biofluid samples enclosing zero biomarkers from those containing just one. The technology can be used for ultimate binary sensing of both proteins/peptides and genomic markers to enable the reliable screening of diseases such as cancer as well as viral and bacterial infections. The NoOne binary platform is designed to be portable, cost-effective, easy to operate and with a time-to-results within one hour; hence it is the ideal candidate for point-of-care applications. The prototype will enable clinicians, phytopathologists or veterinaries, to identify the set of samples that are totally free from a protein, peptide or genomic marker as well as from a pathogen (virus or bacteria), from those enclosing at least one with a confidence level of 99%. This makes the NoOne platform the best performing ever in enabling a fast, highly reliable, cost-effective identification of the subset of biological samples belonging to the potentially diseased part of a population. This is of paramount importance for predictive screening of humans, plants or animals. NoOne will demonstrate its effectiveness in key relevant applications such as the binary detection of pancreatic cancer biomarkers, SARS-CoV-2 virus, the Xylella Fastidiosa bacterium and the assay of post translational peptides evidencing the phosphorylated forms regulating crosstalk with oncogenic signalling pathways.

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Keywords

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Programme(s)

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Topic(s)

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2021-STG

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Host institution

UNIVERSITA DEGLI STUDI DI BARI ALDO MORO
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 969 012,75
Address
PIAZZA UMBERTO I 1
70121 BARI
Italy

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Region
Sud Puglia Bari
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 969 012,75

Beneficiaries (2)

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