Scientists at the European Molecular Biology Laboratory (EMBL) join forces with top scientists from eleven research institutes in Austria, Germany, Italy, France and the United Kingdom for MitoCheck the largest integrated research project on cell cycle control within the European Commissions 6th Framework Programme (FP6). The partners will receive an 8.5 million Euro grant to address a fundamental question: How is cell division regulated? Cell division (or mitosis) is one of the key processes of life. Mistakes during mitosis can cause infertility and mental retardation, and can contribute to cancer. For the most part, mitosis is still poorly understood. Scientists do know that protein kinases a certain type of enzyme play a key role, but researchers dont know how these enzymes bring about the important changes in cells that cause them to divide. To understand cell division in a comprehensive manner, the MitoCheck consortium of European scientists will systematically hunt or all genes that are required for division and then check the products of these genes to see how they are regulated by mitotic kinases. This project is vital to understanding one of the most basic processes of life - making two cells out of one. Here at EMBL, we will identify which genes are required for mitosis by suppressing them gene-by-gene in live human cells and testing whether they can still divide afterwards, notes Jan Ellenberg, EMBL Group Leader and co-initiator of the MitoCheck project. To achieve this, EMBL scientists use a method called RNA interference, where RNA molecules can target and silence specific genes involved in mitosis. Another MitoCheck partner, the Max-Planck Institute of Cell Biology and Genetics in Dresden will provide a vast library of these molecules. About 20,000 genes will be suppressed one-by-one and EMBL researchers will make movies of the cells undergoing division, using sophisticated microscopes developed by Rainer Pepperkok, Jan Ellenberg and Leica Microsystems. Several hundred thousand movies will be produced, filming each group of cells over a 48-hour period, to capture the full impact of silencing particular genes. The task of analyzing the enormous amount of data will be accomplished in a close collaboration of scientists at EMBL and the German Cancer Research Centre (DKFZ). The end result of the work at EMBL will be a comprehensive list of genes required for mitosis in human cells. But most importantly, for he first time, scientists will know exactly which genes are active during mitosis and what happens in the cell when these genes are suppressed. This information will be used by all the partners in this European project to determine the biochemical regulation of mitotic genes, and to test them as tools for cancer diagnosis. All data produced through MitoCheck will be readily available to the scientific community and the public through databases at the DKFZ, and at The Wellcome Trust Sanger Institute, another MitoCheck partner. This project is an excellent example of a European research network, Jan Ellenberg says. We have assembled a group of top scientists across Europe, each of whom is contributing an essential piece towards a common goal - understanding mitosis.About EMBL:,The European Molecular Biology Laboratory is a basic research institute funded by public research monies from 17 member states, including most of the EU, Switzerland and Israel. Research at EMBL is conducted by approximately 80 independent groups covering the spectrum of molecular biology. The Laboratory has five units: the main Laboratory in Heidelberg, and Outstations in Hinxton (the European Bioinformatics Institute), Grenoble, Hamburg, and Monterotondo near Rome. The cornerstones of EMBLs mission are: to perform basic research in molecular biology, to train scientists, students and visitors at all levels, to offer vital services to scientists in the member states, and to develop new instruments and methods in the life sciences. EMBLs international PhD Programme has a student body of about 170. The Laboratory also sponsors an active Science and Society programme. Visitors from the press and public are welcome. For more about MitoCheck and a full list of its participants, please go to: http://www.mitocheck.org.
Austria, Germany, France, Italy, United Kingdom