The growth of intensive aquaculture requires efficient and effective methods to preserve gametes for higher flexibility in broodstock management, genetic improvement programs and preservation of genetic diversity. While methods for cryopreservation of fish spermatozoa are well known, preserving maternally inherited important genetic factors has yet to be achieved. Methods for cryopreservation of yolk-laden fish embryos remain elusive so far, but immature or mature oocytes are a specific challenge.
The objective is to develop technologies for cryopreservation of fish oocytes. Multidisciplinary studies will be performed on the oocyte envelope structure, biological processes during oocyte maturation and hydration, development of in vitro procedures for oocyte maturation, ovulation and fertilization, and on molecular (nucleic acid and protein) markers for indicating oocyte viability prior and after cryopreservation.
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Sant Carles De La R_pita