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Novel bioreductions by hyperthermophilic microorganisms for the natural, specific and on-line production of fine chemicals

Objective

The project aims to exploit the very high reducing power of hyperthermophilic microorganisms for the on-line production of baldheads, (choral) alcohols as well as for the selective reduction of double bonds for a range of industrial applications. The metabolic and genetic diversity of Thermococcales, including pyrococcus and Thermococcus spp. will be exploited by screening available strain collections for specific bio-reducing capacities, as well as the three established Pyrococcus genomes for enzymes catalysing the reductive conversions via genome and protease based approaches. Further development of genetic tools will allow for optimising the in vivo bio reductions at 50-100 °C by metabolic engineering to prevent side reactions. Fermentation conditions and reactor design will be optimised, and on-line product distillation established. Finally, a novel, stable co-factor regenerating system will be applied to the in vitro bio-reduction system.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

WAGENINGEN UNIVERSITY
Address
Bornenweg, 2
6700 EV Wageningen
Netherlands

Participants (8)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
Place Nicolas Copernic
29280 Plouzane
DELFT UNIVERSITY OF TECHNOLOGY
Netherlands
Address
136,Lorentzweg 1
2628 CJ Delft
QUEST INTERNATIONAL B.V.
Netherlands
Address
Huizerstraatweg 28
1400 CA Bussum
STICHTING KATHOLIEKE UNIVERSITEIT
Netherlands
Address
Toernooiveld 1
6525 ED Nijmegen
TUTECH INNOVATION GMBH
Germany
Address
4,Harburger Schlossstrasse 6-12
21079 Hamburg
UNIVERSITE DE BRETAGNE OCCIDENTALE
France
Address
Place Nicolas Copernic
29280 Plouzane
UNIVERSITY OF GRONINGEN
Netherlands
Address
4,Nijenborgh 4
9747 AG Groningen
UNIVERSITY OF SZEGED
Hungary
Address
Dugonicster 13
Szeged